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GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A

GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A

Authors :
Wolfgang Schreibmayer
Ruth Prassl
Stefanie Stanzer
Katja Ester
Sonja Langthaler
Nassim Ghaffari-Tabrizi-Wizsy
Simin Rezania
Andreas Prokesch
Helmut Ahammer
Dieter Platzer
Susanne Scheruebel
Brigitte Pelzmann
Klaus Zorn-Pauly
Gebhard Schratter
Trevor DeVaney
Thomas Bauernhofer
Kurt Schmidt
Astrid Gorischek
Stephan W Jahn
Source :
Scientific Reports, Vol 9, Iss 1, Pp 1-18 (2019), Scientific Reports
Publication Year :
2019
Publisher :
Nature Publishing Group, 2019.

Abstract

Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with the control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, but to a lesser extent. Thus, GIRK1 regulated cellular pathways in mammary epithelial cells are likely to contribute to the development and progression of breast cancer.

Details

Language :
English
ISSN :
20452322
Volume :
9
Issue :
1
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....0f0d014586551eca02950fc98bfd08de
Full Text :
https://doi.org/10.1038/s41598-019-55683-w