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GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A
GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-18 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with the control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, but to a lesser extent. Thus, GIRK1 regulated cellular pathways in mammary epithelial cells are likely to contribute to the development and progression of breast cancer.
- Subjects :
- Cell biology
Carcinogenesis
Molecular biology
Biophysics
lcsh:Medicine
Breast Neoplasms
Diseases
Biology
Biochemistry
Article
GIRK1
breast cancer
MCF10A
MCF7
Transcriptome
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
Breast cancer
Medical research
Cell Movement
Neoplasms
medicine
Humans
Mammary Glands, Human
skin and connective tissue diseases
lcsh:Science
030304 developmental biology
Cancer
Regulation of gene expression
0303 health sciences
Multidisciplinary
Molecular medicine
lcsh:R
Cell migration
medicine.disease
3. Good health
Gene Expression Regulation, Neoplastic
G Protein-Coupled Inwardly-Rectifying Potassium Channels
Oncology
030220 oncology & carcinogenesis
Lymphatic Metastasis
Cancer research
MCF-7 Cells
Female
lcsh:Q
Wound healing
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....0f0d014586551eca02950fc98bfd08de
- Full Text :
- https://doi.org/10.1038/s41598-019-55683-w