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Identification of a Novel Geminivirus in Fraxinus rhynchophylla in Korea
- Source :
- Viruses; Volume 13; Issue 12; Pages: 2385, Viruses, Vol 13, Iss 2385, p 2385 (2021), Viruses
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Fraxinus rhynchophylla, common name ash, belongs to the family Oleaceae and is found in China, Korea, North America, the Indian subcontinent, and eastern Russia. It has been used as a traditional herbal medicine in Korea and various parts of the world due to its chemical constituents. During a field survey in March 2019, mild vein thickening (almost negligible) was observed in a few ash trees. High-throughput sequencing of libraries of total DNA from ash trees, rolling-circle amplification (RCA), and polymerase chain reaction (PCR) allowed the identification of a Fraxinus symptomless virus. This virus has five confirmed open reading frames along with a possible sixth open reading frame that encodes the movement protein and is almost 2.7 kb in size, with a nonanucleotide and stem loop structure identical to begomoviruses. In terms of its size and structure, this virus strongly resembles begomoviruses, but does not show any significant sequence identity with them. To confirm movement of the virus within the trees, different parts of infected trees were examined, and viral movement was successfully observed. No satellite molecules or DNA B were identified. Two-step PCR confirmed the virion and complementary strands during replication in both freshly collected infected samples of ash tree and Nicotiana benthamiana samples agro-inoculated with infectious clones. This taxon is so distantly grouped from other known geminiviruses that it likely represents a new geminivirus genus.
- Subjects :
- Fraxinus symptomless virus
geminivirus
Nicotiana benthamiana
Genome, Viral
Fraxinus
Microbiology
Article
Virus
law.invention
Open Reading Frames
law
Virology
Republic of Korea
Tobacco
Botany
Movement protein
Phylogeny
Polymerase chain reaction
Plant Diseases
Base Sequence
biology
begomovirus
Fraxinus rhynchophylla
Begomovirus
biology.organism_classification
QR1-502
Open reading frame
Geminiviridae
Infectious Diseases
DNA, Viral
Satellite (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses; Volume 13; Issue 12; Pages: 2385
- Accession number :
- edsair.doi.dedup.....0f107282bdc76392fad84868332fb0be
- Full Text :
- https://doi.org/10.3390/v13122385