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Elevated expression of mitochondrial transcription elongation factor (TEFM) predicts poor prognosis in low grade glioma—an analysis of the Cancer Genome Atlas (TCGA) dataset
- Source :
- Translational Cancer Research
- Publication Year :
- 2020
- Publisher :
- AME Publishing Company, 2020.
-
Abstract
- Background Mitochondrial transcription elongation factor (TEFM) is a key molecule for mitochondrial DNA (mtDNA) replication-transcription switch. TEFM regulates both transcription elongation and RNA processing in mitochondria. However, the expression level and prognostic value of TEFM in low grade glioma (LGG) remain unclear. Therefore, in this study, we aimed to evaluate the clinical significance and the prognostic value of TEFM in LGG based on publicly available data. Methods The relative mRNA expression level of TEFM in non-tumor brain tissues and LGG tissues were retrieved from Gene Expression Profiling Interactive Analysis (GEPIA). The RNA-Seq expression of TEFM and clinical information in LGG patients were collected from the updated the Cancer Genome Atlas (TCGA) database by using R3.6.1 software. Next, the relationship between the mRNA expression of TEFM and clinicopathological characteristics were analyzed. Kaplan-Meier survival curves of overall survival (OS) and disease-free survival (DFS) were implemented for the relationship between the mRNA expression of TEFM and the prognosis of LGG patients. A Cox regression model was performed for the multivariate analysis of the factors affected the prognosis of LGG patients. GEPIA online tool was used to analyze the correlation between TEFM gene expression level and other related mitochondrial regulatory genes in LGG. Finally, The Gene Set Enrichment Analysis (GSEA) was performed to identify cell processes and molecular signaling cascades affected by TEFM. Results GEPIA analysis showed that the mRNA expression levels of TEFM in LGG were significantly higher than that of non-tumor tissue. Moreover, the mRNA expression of TEFM is significantly correlated with age, World Health Organization (WHO) grade, pathological types, headache history and supratentorial location (P0), but negatively correlated with the POLRMT gene (P
- Subjects :
- Cancer Research
Poor prognosis
Biology
Elongation factor
medicine.anatomical_structure
Oncology
Atlas (anatomy)
Cancer genome
Mitochondrial transcription
medicine
Cancer research
Original Article
The Cancer Genome Atlas dataset (TCGA dataset)
Radiology, Nuclear Medicine and imaging
Low-Grade Glioma
prognosis
mitochondrial transcription elongation factor (TEFM)
low grade glioma (LGG)
Subjects
Details
- ISSN :
- 22196803 and 2218676X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Translational Cancer Research
- Accession number :
- edsair.doi.dedup.....0f1c4e826ed5b0b8672af6e5e01d9496