Disposition and metabolism of 2′,2′'-Dithiobisbenzanilide in rodents following intravenous and oral administration and dermal application

Highlights • 2′,2′′′-Dithiobisbenzanilide (DTBBA) is a chemical used as a peptizing agent for rubber. • Humane exposure to DTBBA is possible via oral and dermal routes. • DTBBA is well-absorbed in rodents following oral and dermal administration. • Absorbed DTBBA was extensively metabolized and excreted mainly via urine. • N-(2-mercaptophenyl)benzamide accounted for more than 50% of radioactivity in urine.
2′,2′′′-Dithiobisbenzanilide (DTBBA) is a high-production-volume chemical used as a peptizing agent for rubber. The disposition and metabolism of [14C]DTBBA were determined in male and female rats and mice following oral (4, 40, or 400 mg/kg) and intravenous (IV) (4 mg/kg) administration and dermal application (0.4 or 4 mg/kg). [14C]DTBBA was well absorbed following oral administration (> 60%) and dermal application (∼40–50%) in rats and mice. Following oral administration, the majority of radioactivity was excreted in urine (29 − 70%) and feces (16 − 45%). Unlike rats, mice excreted ∼1-5% of the dose as exhaled CO2. The residual radioactivity in tissues was