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Anti-Inflammatory Effects of Dietary Plant Stanol Supplementation Are Largely Dependent on the Intake of Cholesterol in a Mouse Model of Metabolic Inflammation

Authors :
Jogchum Plat
Dieter Lütjohann
Ronit Shiri-Sverdlov
Marion J.J. Gijbels
Tom Houben
Inês Magro dos Reis
Medical Biochemistry
ACS - Atherosclerosis & ischemic syndromes
AII - Inflammatory diseases
Moleculaire Genetica
RS: NUTRIM - R2 - Liver and digestive health
Pathologie
RS: Carim - B07 The vulnerable plaque: makers and markers
Nutrition and Movement Sciences
RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health
Source :
Biomedicines, Biomedicines, Vol 9, Iss 518, p 518 (2021), Biomedicines, 9(5):518. MDPI AG, Volume 9, Issue 5
Publication Year :
2021
Publisher :
MDPI, 2021.

Abstract

The prevalence of metabolic disorders characterized by chronic inflammation has been on a sharp rise for decades. As such, tools that address metabolic and inflammatory dysregulation are of great importance. Plant stanols are well-known for reducing intestinal cholesterol absorption and may also have direct anti-inflammatory effects. In this study, our aim was to investigate to what extent the benefits of dietary plant stanol supplementation depend on dietary cholesterol intake in an experimental mouse model for cholesterol-induced metabolic inflammation. Here, we used Ldlr−/− mice transplanted with Npc1nih-derived bone marrow, featuring feature bone marrow-derived immune cells characterized by chronic inflammation induced by lysosomal lipid accumulation. Npc1nih- and Npc1wt-transplanted mice were placed on either a high fat, high cholesterol (HFC) or on a chow diet low in cholesterol, with or without 2% plant stanols supplementation. At the end of the study, the metabolic and inflammatory status of the mice was analyzed. Plant stanol supplementation to the HFC diet reduced liver cholesterol levels and improved lipid metabolism and liver inflammation, particularly in Npc1nih-tp mice. In contrast, plant stanol supplementation to the chow diet did not significantly improve the aforementioned parameters, though similar reductive trends to those in the HFC diet setting were observed regarding liver cholesterol accumulation and liver inflammatory markers. The effects of dietary plant stanol supplementation on dietary cholesterol-induced inflammation are largely dependent on dietary cholesterol intake. Future research should verify whether other models of metabolic inflammation exhibit similar stanol-related effects on inflammation.

Details

Language :
English
ISSN :
22279059
Volume :
9
Issue :
5
Database :
OpenAIRE
Journal :
Biomedicines
Accession number :
edsair.doi.dedup.....0f7965a10d74167faf05c3b655db0b18