Back to Search
Start Over
STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta
- Source :
- The EMBO Journal. 22:893-904
- Publication Year :
- 2003
- Publisher :
- Wiley, 2003.
-
Abstract
- Transcriptional activation is associated commonly with recruitment of histone acetylases, while repression involves histone deacetylases (HDACs). Here, we provide evidence to suggest that STAT5 activates gene expression by recruiting HDAC. The interleukin-3 (IL-3)-dependent expression of the Id-1 gene, encoding a helix-loop-helix (HLH) transcriptional inhibitor, is activated by both C/EBPbeta and STAT5 transcription factors bound to its pro-B-cell enhancer (PBE), but is inhibited by HDAC inhibitors in Ba/F3 cells. STAT5 interacts with HDAC1 in the PBE region, resulting in deacetylation of histones, as well as C/EBPbeta, whose acetylation diminishes its DNA-binding activity. Consistently, expression of an acetylation-resistant mutant of C/EBPbeta results in IL-3-independent expression of the Id-1 gene. Thus, we propose a novel mechanism by which STAT5 mediates the deacetylation of C/EBPbeta, allowing transcriptional activation.
- Subjects :
- Inhibitor of Differentiation Protein 1
Transcription, Genetic
Histone Deacetylase 1
Biology
DNA-binding protein
Histone Deacetylases
General Biochemistry, Genetics and Molecular Biology
Cell Line
Transcription (biology)
Gene expression
STAT5 Transcription Factor
Animals
Humans
Enhancer
Molecular Biology
Transcription factor
General Immunology and Microbiology
CCAAT-Enhancer-Binding Protein-beta
General Neuroscience
Acetylation
Articles
Milk Proteins
Molecular biology
HDAC1
DNA-Binding Proteins
Repressor Proteins
Enhancer Elements, Genetic
Histone
Trans-Activators
biology.protein
Transcription Factors
Subjects
Details
- ISSN :
- 14602075
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....0fa1fec04dcd7fe7c1035882d229c13c