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In vivo stimulation of polymeric Ig receptor transcytosis by circulating polymeric IgA in rat liver
- Source :
- International immunology. 10(3)
- Publication Year :
- 1998
-
Abstract
- Binding of human polymeric IgA ligand to its epithelial cell polymeric Ig receptor, pIgR, has been shown to stimulate pIgR apical transcytosis in an in vitro system, based on polarized confluent MDCK cells expressing rabbit pIgR. The present study aimed at testing whether such a stimulation also occurs in vivo. Transcytosis of pIgR was monitored by rat liver output of total secretory component (SC) into bile, measured by radial immunodiffusion as the sum of free SC and pIgA-bound SC. Whereas in the perfused rat liver system addition of pIgA to the perfusate showed no effect, i.v. injection of human and rat pIgA, but not of monomeric IgA nor PBS, in living rats significantly increased total bile SC output for more than 1 h. Furthermore, depletion of the normal pIgA level circulating in the liver before injecting more pIgA was not required to show the stimulation. Our data thus strongly suggest that stimulation of liver pIgR transcytosis by pIgA ligand binding is physiologically relevant, helping to quickly adjust pIgA transport into bile to increase circulating pIgA levels, without need for increased SC/pIgR synthesis.
- Subjects :
- Immunoglobulin A
Male
medicine.medical_specialty
Secretory component
Immunology
Stimulation
Rats, Sprague-Dawley
In vivo
Internal medicine
medicine
Immunology and Allergy
Animals
Receptor
biology
Receptors, Polymeric Immunoglobulin
Biological Transport
General Medicine
Ligand (biochemistry)
Epithelium
Cell biology
Rats
Perfusion
Endocrinology
medicine.anatomical_structure
Transcytosis
Liver
biology.protein
Subjects
Details
- ISSN :
- 09538178
- Volume :
- 10
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International immunology
- Accession number :
- edsair.doi.dedup.....0ff628938cbef2379df65d5e7476ea44