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Lipid response patterns in acute phase paediatric Plasmodium falciparum malaria
- Source :
- Metabolomics. 13
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
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Abstract
- Introduction: Several studies have observed serum lipid changes during malaria infection in humans. All of them were focused at analysis of lipoproteins, not specific lipid molecules. The aim of our study was to identify novel patterns of lipid species in malaria infected patients using lipidomics profiling, to enhance diagnosis of malaria and to evaluate biochemical pathways activated during parasite infection. Methods: Using a multivariate characterization approach, 60 samples were representatively selected, 20 from each category (mild, severe and controls) of the 690 study participants between age of 0.5–6 years. Lipids from patient’s plasma were extracted with chloroform/methanol mixture and subjected to lipid profiling with application of the LCMS-QTOF method. Results: We observed a structured plasma lipid response among the malaria-infected patients as compared to healthy controls, demonstrated by higher levels of a majority of plasma lipids with the exception of even-chain length lysophosphatidylcholines and triglycerides with lower mass and higher saturation of the fatty acid chains. An inverse lipid profile relationship was observed when plasma lipids were correlated to parasitaemia. Conclusions: This study demonstrates how mapping the full physiological lipid response in plasma from malaria-infected individuals can be used to understand biochemical processes during infection. It also gives insights to how the levels of these molecules relate to acute immune responses.
- Subjects :
- 0301 basic medicine
Infectious Medicine
Endocrinology, Diabetes and Metabolism
Plasmodium falciparum
Triacylglycerides
030106 microbiology
Clinical Biochemistry
Infektionsmedicin
Biochemistry
03 medical and health sciences
parasitic diseases
Lipidomics
medicine
Parasite hosting
biology
business.industry
Lysophosphatidylcholines
medicine.disease
biology.organism_classification
Molecular medicine
Malaria
Diagnosis of malaria
030104 developmental biology
Immunology
lipids (amino acids, peptides, and proteins)
business
Lipidomics profiling
Subjects
Details
- ISSN :
- 15733890 and 15733882
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Metabolomics
- Accession number :
- edsair.doi.dedup.....103722e858225f1a1ac13b83442634e0