Back to Search
Start Over
Plasma <scp>miR</scp> ‐409‐3p promotes acute cerebral infarction via suppressing <scp>CTRP3</scp>
- Source :
- Kaohsiung Journal of Medical Sciences, Vol 37, Iss 4, Pp 324-333 (2021)
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Abnormal expression of miR‐409‐3p has been found in several neurodevelopmental disorders, but whether it is dysregulated in the patients with acute cerebral infarction (ACI) has not been evaluated. The current study mainly focused on the clinical significance and the underlying mechanism of plasma miR‐409‐3p in the progression of ACI. The level of plasma miR‐409‐3p was determined in ACI patients (n = 80) and healthy controls (n = 30). Pearson correlation assay was performed to evaluate the association and cardiovascular risk factors. A receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of plasma miR‐409‐3p levels in patients with ACI. Dual luciferase reporter assay and western blot were performed to determine the possible target gene of miR‐409‐3p. Our data showed that the expression of plasma miR‐409‐3p in the ACI group was higher than that in the healthy controls. Furthermore, Pearson correlation analysis indicated a positive correlation between plasma miR‐409‐3p and the NIHSS score. ROC analysis indicated that plasma miR‐409‐3p could differentiate plasma miR‐409‐3p in ACI patients from healthy controls. Then, we explored the possible target genes of miR‐409‐3p. Interestingly, C1q and TNF‐related 3 (CTRP3), a novel adipose tissue‐derived secreted factor, was found to be a target gene of miR‐409‐3p. We found that knockdown of CTRP3 significantly induced PC12 cell apoptosis, even in PC12 cells transfected with miR‐409‐3p inhibitor. These data suggested that miR‐409‐3p induced PC12 cell apoptosis by targeting CTRP3. Altogether, elevated plasma miR‐409‐3p is correlated with disease severity and may be efficient for the early diagnosis of ACI.
- Subjects :
- Adult
Male
medicine.medical_specialty
miR‐409‐3p
education
Adipose tissue
PC12 Cells
03 medical and health sciences
0302 clinical medicine
Western blot
Genes, Reporter
Risk Factors
Internal medicine
medicine
Animals
Humans
Clinical significance
Luciferases
Gene
Aged
lcsh:R5-920
Gene knockdown
medicine.diagnostic_test
Receiver operating characteristic
business.industry
apoptosis
Cerebral Infarction
General Medicine
Transfection
Middle Aged
Rats
MicroRNAs
acute cerebral infarction
Endocrinology
Gene Expression Regulation
ROC Curve
Apoptosis
Case-Control Studies
030220 oncology & carcinogenesis
Acute Disease
Tumor Necrosis Factors
Disease Progression
Female
030211 gastroenterology & hepatology
lcsh:Medicine (General)
business
Biomarkers
CTRP3
Signal Transduction
Subjects
Details
- ISSN :
- 24108650 and 1607551X
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- The Kaohsiung Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....105bb46e4159231c24fbc1bdfc6143bc