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Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts e Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring

Authors :
Henner Hollert
Adam Jonáš
Magnus Breitholtz
Pascal Pandard
Tobias Schulze
Nick Zwart
Sergio Jarque
Carole Chalon
Steffen Keiter
Werner Brack
Andrea Schifferli
Carolina Di Paolo
Emmanuelle Maillot-Marechal
Thomas-Benjamin Seiler
Kerstin Bluhm
Richard Ottermanns
Beate I. Escher
Valeria Dulio
Sebastian Buchinger
Yves Marneffe
Ron van der Oost
Radka Zounková
Selim Ait-Aissa
Xavier Cousin
Janet Y. M. Tang
Klára Hilscherová
Etiënne L.M. Vermeirssen
Mai Thao Nguyen
Timo Hamers
Sven Seidensticker
Mario Carere
Chemistry and Biology
Amsterdam Global Change Institute
Institute for Environmental Research
Rheinisch-Westfälische Technische Hochschule Aachen (RWTH)
Man-Technology-Environment Research Centre, School of Science and Technology
Örebro University
Institut National de l'Environnement Industriel et des Risques (INERIS)
Helmholtz Zentrum für Umweltforschung = Helmholtz Centre for Environmental Research (UFZ)
Department of Applied Environmental Science [Stockholm] (ITM)
Stockholm University
Department Biochemistry and Ecotoxicology
Federal Institute of Hydrology
Italian Institute of Health
Scientific Institute of Public Service (ISSeP)
Laboratoire de Physiologie et Génomique des Poissons (LPGP)
Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Laboratoire d'Ecotoxicologie
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
National Research Centre for Environmental Toxicology
University of Southern Queensland (USQ)
Center for Applied Geosciences [Tübingen] (ZAG)
University of Tübingen
Institute for Environmental Studies
University of Amsterdam [Amsterdam] (UvA)
Research Centre for Toxic Compounds in the Environment [Brno] (RECETOX / MUNI)
Faculty of Science [Brno] (SCI / MUNI)
Masaryk University [Brno] (MUNI)-Masaryk University [Brno] (MUNI)
Waterproef Laboratory
Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG)
Division of Technology Research & Engineering
Waternet Institute for the Urban Water Cycle
Institute for Environmental Studies -IVM
This project was developed by the Working Group 2 Bioassays and biomarkers in water quality monitoring of the NORMAN Association ( http://www.norman-network.net ). It was funded and supported by the European Marie Curie Initial Training Network EDA-EMERGE (grant agreement no. 290100
Helmholtz Centre for Environmental Research (UFZ)
University of Queensland (UQ)
Research Centre for Toxic Compounds in the Environment
Masaryk University
Swiss Centre for Applied Ecotoxicology Eawag-EPFL
Source :
Water Research, 104, 473-484. Elsevier Limited, Di Paolo, C, Ottermanns, R, Keiter, S, Ait-Aissa, S, Bluhm, K, Brack, W, Breitholz, M, Buchinger, S, Carere, M, Chalon, C, Cousin, X, Dulio, V, Escher, B I, Hamers, T, Jarque, S, Jonas, A, Maillot-Marechal, E, Marneffe, Y, Nguyen, M T, Pandard, P, Schifferli, A, Schulze, T, Seidensticker, S, Seiler, T B, Tang, J, van der Oost, R, Vermeirssen, E, Zounková, R, Zwart, N & Hollert, H 2016, ' Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts e Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring ', Water Research, vol. 104, pp. 473-484 . https://doi.org/10.1016/j.watres.2016.08.018, Water Research, Water Research, IWA Publishing, 2016, 104, pp.473-484. ⟨10.1016/j.watres.2016.08.018⟩, Water Research (0043-1354) (Pergamon-elsevier Science Ltd), 2016-11, Vol. 104, P. 473-484
Publication Year :
2016

Abstract

Bioassays are particularly useful tools to link the chemical and ecological assessments in water quality monitoring. Different methods cover a broad range of toxicity mechanisms in diverse organisms, and account for risks posed by non-target compounds and mixtures. Many tests are already applied in chemical and waste assessments, and stakeholders from the science-police interface have recommended their integration in regulatory water quality monitoring. Still, there is a need to address bioassay suitability to evaluate water samples containing emerging pollutants, which are a current priority in water quality monitoring. The presented interlaboratory study (ILS) verified whether a battery of miniaturized bioassays, conducted in 11 different laboratories following their own protocols, would produce comparable results when applied to evaluate blinded samples consisting of a pristine water extract spiked with four emerging pollutants as single chemicals or mixtures, i.e. triclosan, acridine, 17 alpha-ethinylestradiol (EE2) and 3-nitrobenzanthrone (3-NBA). Assays evaluated effects on aquatic organisms from three different trophic levels (algae, daphnids, zebrafish embryos) and mechanism-specific effects using in vitro estrogenicity (ER-Luc, YES) and mutagenicity (Ames fluctuation) assays. The test battery presented complementary sensitivity and specificity to evaluate the different blinded water extract spikes. Aquatic organisms differed in terms of sensitivity to triclosan (algae > daphnids > fish) and acridine (fish > daphnids > algae) spikes, confirming the complementary role of the three taxa for water quality assessment. Estrogenicity and mutagenicity assays identified with high precision the respective mechanism-specific effects of spikes even when non-specific toxicity occurred in mixture. For estrogenicity, although differences were observed between assays and models, EE2 spike relative induction EC50 values were comparable to the literature, and E2/EE2 equivalency factors reliably reflected the sample content. In the Ames, strong revertant induction occurred following 3-NBA spike incubation with the TA98 strain, which was of lower magnitude after metabolic transformation and when compared to TA100. Differences in experimental protocols, model organisms, and data analysis can be sources of variation, indicating that respective harmonized standard procedures should be followed when implementing bioassays in water monitoring. Together with other ongoing activities for the validation of a basic bioassay battery, the present study is an important step towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring. (C) 2016 Elsevier Ltd. All rights reserved.

Details

Language :
English
ISSN :
00431354
Volume :
104
Database :
OpenAIRE
Journal :
Water Research
Accession number :
edsair.doi.dedup.....105c25256af0df70e402188f471be5c0
Full Text :
https://doi.org/10.1016/j.watres.2016.08.018