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Involvement between social defeat stress and pain-related behavior in a rat lumbar disk herniation model
- Source :
- European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society. 29(10)
- Publication Year :
- 2020
-
Abstract
- Psychological and social factors are involved in the disability and chronicity of pain. Our study aim was to investigate whether social defeat stress (SDS) as a psychophysical stress affected mechanical withdrawal thresholds in the lumbar disk herniation (LDH) rat model. Changes in microglia and astrocytes, which play important roles in neuropathic pain states, were also investigated. For the LDH model, nucleus pulposus (NP) was applied to the L5 dorsal root ganglion (DRG) in adult female Sprague–Dawley rats. SDS was performed 15 min daily for 8 days. Mechanical withdrawal thresholds were measured, and immunoreactive cells of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule-1 (Iba-1), which were used as markers of microglia, satellite glial cells, and astrocytes, were assessed in the DRG, spinal cord (SC), and ventrolateral periaqueductal gray matter (VLPAG). Mechanical withdrawal thresholds decreased in the NP group for 21 days and for 35 days in the NP + SDS group. Expression of GFAP and Iba-1 in the DRG and SC increased up to day 21 in the NP and NP + SDS groups. In the sham + SDS and NP + SDS groups, expression of GFAP in the VLPAG decreased until day 35. SDS prolongs mechanical allodynia induced by NP. Changes of GFAP expression in the VLPAG were associated with mechanical allodynia of the NP + SDS group during the late phase. These results suggest that psychological chronic stress might delay recovery from mechanical allodynia induced by the LDH model.
- Subjects :
- medicine.medical_specialty
Intervertebral Disc Degeneration
Social defeat
Rats, Sprague-Dawley
Social Defeat
03 medical and health sciences
0302 clinical medicine
Lumbar
Dorsal root ganglion
Internal medicine
Ganglia, Spinal
medicine
Animals
Orthopedics and Sports Medicine
Chronic stress
030222 orthopedics
Lumbar Vertebrae
Microglia
Glial fibrillary acidic protein
biology
business.industry
Spinal cord
Rats
Disease Models, Animal
medicine.anatomical_structure
Endocrinology
Hyperalgesia
Neuropathic pain
biology.protein
Surgery
Female
business
030217 neurology & neurosurgery
Intervertebral Disc Displacement
Subjects
Details
- ISSN :
- 14320932
- Volume :
- 29
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
- Accession number :
- edsair.doi.dedup.....109d891ceb3c1959a5af72671bff90d3