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A cDNA for European sea bass (Dicentrachus labrax) 11 beta-hydroxylase: Gene expression during the thermosensitive period and gonadogenesis
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2007
- Publisher :
- Elsevier, 2007.
-
Abstract
- Steroid P450 11beta-hydroxylase, encoded by the CYP11B gene, is a key mitochondrial enzyme for the production of 11-oxygenated androgens, which have been shown to be potent masculinising steroids in several fish species. In this study we have isolated a CYP11B cDNA of 1903 base pairs from the testis of European sea bass (Dicentrarchus labrax) encoding a predicted protein of 552 amino acids. The amino acid identities to other vertebrate 11beta-hydroxylase proteins ranged from 66% to 72% to other fish; 45% to amphibian and 35-39% to mammalian. Southern blot indicated that a single CYP11B gene is present. Northern blot analysis detected two transcripts in testis and head kidney, one of the same size of the isolated clone and the other of 3.9 kb. Reverse transcriptase-polymerase chain reaction showed abundant mRNA expression only in testis and head kidney, being residual in a range of other tissues. Expression of CYP11B and CYP19A (which encodes for ovarian aromatase) was detected from at least 4 days post-hatching and did not appear to be affected by rearing temperature (15 and 20 degrees C) during the first 60 days, a period in which high temperatures promote masculinisation in European sea bass. Throughout, gonadogenesis (60-300 dph), a highly dimorphic pattern of CYP11B expression was consistent with a role of this gene in testicular development.
- Subjects :
- Male
food.ingredient
DNA, Complementary
Sex Differentiation
Organogenesis
Molecular Sequence Data
Bass (fish)
Endocrinology
food
Aromatase
Complementary DNA
Testis
Animals
Dicentrarchus labrax
Northern blot
RNA, Messenger
Steroid 11-beta-hydroxylase
Sea bass
Gene
Southern blot
11 -Hydroxylase
biology
Base Sequence
CYP11B
Temperature
Gene Expression Regulation, Developmental
biology.organism_classification
Molecular biology
Androgens
Steroid 11-beta-Hydroxylase
Animal Science and Zoology
Dicentrarchus
Bass
Female
Sex diVerentiation
CYP19A
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....10f2e5d260c6bea479772a036f270b69