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Global quality assessment of liver allograft C4d staining during acute antibody-mediated rejection in formalin-fixed, paraffin-embedded tissue
- Source :
- Neil, D A H, Bellamy, C, Smith, M, Haga, H, Yoh, Z, Sebagh, M, Ruppert, K, Lunz, J G, Hubscher, S & Demetris, A J 2017, ' Global quality assessment of liver allograft C4d staining during acute antibody mediated rejection in formalin-fixed paraffin-embedded tissue ', Human pathology . https://doi.org/10.1016/j.humpath.2017.12.007
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Discussion of liver antibody-mediated rejection during the 2011, 2013, and 2015 Banff liver sessions raised concerns over reliability of complement fragment 4d (C4d) staining, precipitating a global survey followed by a tissue microarray staining quality assessment study among centers on formalin-fixed, paraffin-embedded tissue. Tissue microarray sections containing tissue plugs of resected native and allograft (with acute antibody-mediated rejection) liver, heart, and kidney (n = 33 total cores) were sent to 31 centers for C4d staining using local method(s) and pathologist scoring. Digital whole-slide images (n = 40) were then semiquantitatively scored by 7 experts for background, distribution, and intensity of portal vein and capillary, hepatic artery, sinusoidal, and central vein endothelia and portal and central stromal staining. Results showed that strong and diffuse portal vein and capillary C4d staining, as determined by both local and central pathologists, clearly distinguished allografts showing acute antibody-mediated rejection from native livers and from those with evidence of weaker donor-specific antibody. Downstream vascular endothelial cell C4d staining and assessment were more variable and difficult to identify. C4d staining in the majority of laboratories reliably detects acute liver allograft antibody-mediated rejection in formalin-fixed, paraffin-embedded tissues. Assessment should focus on portal veins and capillaries, sinusoids, and central veins present in peripheral core needle biopsies. C4d staining in one organ does not always translate to staining in another.
- Subjects :
- Graft Rejection
Pathology
medicine.medical_specialty
Tissue Fixation
Stromal cell
030230 surgery
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Formaldehyde
Complement C4b
medicine
Humans
Vein
Kidney
Paraffin Embedding
Tissue microarray
Staining and Labeling
biology
business.industry
Reproducibility of Results
Allografts
Immunohistochemistry
Peptide Fragments
Liver Transplantation
Staining
Endothelial stem cell
medicine.anatomical_structure
Tissue Array Analysis
biology.protein
030211 gastroenterology & hepatology
Antibody
business
Artery
Subjects
Details
- ISSN :
- 00468177
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Human Pathology
- Accession number :
- edsair.doi.dedup.....113cd9284c6812b90ba41cc9d8085a68