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A novel 21-kDa cytochrome c-releasing factor is generated upon treatment of human leukemia U937 cells with geranylgeraniol
- Source :
- Biochemical and biophysical research communications. 346(2)
- Publication Year :
- 2006
-
Abstract
- Geranylgeraniol (GGO) induces apoptosis in various lines of human tumor cells through a mitochondrion-dependent pathway. The present study describes identification of a 21-kDa cytochrome c-releasing factor that appears in the cytosolic fraction after treatment of human leukemia U937 cells with GGO. Incubation of isolated mitochondria with a lysate of U937 cells that had been treated with GGO resulted in the release of cytochrome c from the mitochondria. Utilizing this cell-free system, we purified a 21-kDa protein that induced the release of cytochrome c from mitochondria and appeared to be involved in the apoptosis that is induced in U937 cells by GGO. We designated this protein cytochrome c-releasing factor 21 (CRF21). Overexpression of CRF21 in HeLa cells induced the release of cytochrome c from mitochondria, with subsequent apoptosis. Our results suggest that CRF21 might play an important role in the induction of apoptosis by GGO in leukemia U937 cells.
- Subjects :
- Cytochrome
Biophysics
Apoptosis
Mitochondrion
Biochemistry
HeLa
Mitochondrial Proteins
chemistry.chemical_compound
Geranylgeraniol
medicine
Humans
Amino Acid Sequence
Molecular Biology
biology
Cytochrome c
Cytochromes c
Cell Biology
U937 Cells
medicine.disease
biology.organism_classification
Molecular biology
Cell biology
Mitochondria
Leukemia
chemistry
biology.protein
Apoptosome
Diterpenes
HeLa Cells
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 346
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....11433006a1664c8ab82daa510d9062f0