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Development and characterization of a novel luciferase based cytotoxicity assay
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- A simple, accurate, sensitive and robust assay that can rapidly and specifically measure the death of target cells would have applications in many areas of biomedicine and particularly for the development of novel cellular- and immune-therapeutics. In this study, we describe a novel cytotoxicity assay, termed the Matador assay, which takes advantage of the extreme brightness, stability and glow-like characteristics of recently discovered novel marine luciferases and their engineered derivatives. The assay involves expression of a luciferase of interest in target cells in a manner so that it is preferentially retained within the healthy cells but is either released from dead and dying cells or whose activity can be preferentially measured in dead and dying cells. We demonstrate that this assay is highly sensitive, specific, rapid, and can be performed in a single-step manner without the need for any expensive equipment. We further validate this assay by demonstrating its ability to detect cytotoxicity induced by several cellular and immune-therapeutic agents including antibodies, natural killer cells, chimeric antigen receptor expressing T cells and a bispecific T cell engager.
- Subjects :
- 0301 basic medicine
T cell
lcsh:Medicine
Article
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Toxicity Tests
medicine
Humans
Luciferase
lcsh:Science
Luciferases
Cytotoxicity
Cells, Cultured
Multidisciplinary
biology
Chemistry
lcsh:R
HEK 293 cells
Chimeric antigen receptor
3. Good health
Cell biology
HEK293 Cells
030104 developmental biology
medicine.anatomical_structure
Cell culture
030220 oncology & carcinogenesis
biology.protein
lcsh:Q
Antibody
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....118ef95b9f2a94ee6cc9a1b73ea63b38