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HPLC Analysis of Rosmarinic Acid in Feed Enriched with Aerial Parts of Prunella vulgaris and Its Metabolites in Pig Plasma Using Dual-Channel Coulometric Detection
- Source :
- Journal of Agricultural and Food Chemistry. 55:7631-7637
- Publication Year :
- 2007
- Publisher :
- American Chemical Society (ACS), 2007.
-
Abstract
- This paper describes a sensitive isocratic HPLC/ECD method developed for the determination of rosmarinic acid (RA) in plant material, animal feed, and pig plasma. The plasma sample preparation only includes protein precipitation and adjustment of the pH. The applicability of the method was tested on plasma samples of pigs that were exposed to a 91-day oral intake of RA via feed enriched by aerial parts of Prunella vulgaris. The plasma was directly analyzed using the method described as well as after enzymatic hydrolysis. When no hydrolysis step was included, RA and caffeic acid (CA) were quantified in the plasma. In hydrolyzed plasma samples, several other metabolites were determined, including dihydrocaffeic, ferulic, and dihydroferulic acid. The dual-channel coulometric detection employed, as an alternative to mass spectrometry, offers good selectivity and sensitivity owing to the electrochemical properties of the phenolic constituents.
- Subjects :
- Chromatography
biology
Swine
Rosmarinic acid
Metabolite
Prunella vulgaris
General Chemistry
biology.organism_classification
Animal Feed
Depsides
Sensitivity and Specificity
High-performance liquid chromatography
chemistry.chemical_compound
Hydrolysis
chemistry
Cinnamates
Enzymatic hydrolysis
Food, Fortified
Caffeic acid
Animals
Protein precipitation
Prunella
General Agricultural and Biological Sciences
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....11a34d769296106481baec0a2d81c0da
- Full Text :
- https://doi.org/10.1021/jf070883e