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Screening and identification of a novel B-cell neutralizing epitope from Helicobacter pylori UreB
- Source :
- Vaccine. 26:6945-6949
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Urease plays a crucial role in the survival and pathogenesis of Helicobacter pylori (H. pylori), and antibody neutralizing the urease activity may be implicated for the protection against H. pylori infection. Previously, a neutralizing monoclonal antibody (MAb) 6E6 against UreB of H. pylori was developed. In this work, we try to identify the B-cell epitope recognized by neutralizing MAb 6E6. Following screening a series of truncated proteins of UreB, an epitope was primarily localized in the aa 200–230 of UreB. Subsequently, we screened the overlapping synthetic peptides covering the aa 200–230 and identified a novel B-cell epitope (U211–225, IEAGAIGFKIHEDWG) that was recognized by specific MAb 6E6. The newly identified epitope may help understanding of the protective immunity against H. pylori and be implicated for vaccine development. © 2008 Published by Elsevier Ltd.
- Subjects :
- Models, Molecular
Protein Conformation
medicine.drug_class
Protein subunit
Spirillaceae
Blotting, Western
Drug Evaluation, Preclinical
Molecular Conformation
Enzyme-Linked Immunosorbent Assay
Monoclonal antibody
Epitope
Helicobacter Infections
Microbiology
Pathogenesis
Epitopes
Mice
Neutralization Tests
medicine
Animals
Antibodies, Blocking
Neutralizing antibody
B cell
B-Lymphocytes
Mice, Inbred BALB C
Helicobacter pylori
General Veterinary
General Immunology and Microbiology
biology
Public Health, Environmental and Occupational Health
Antibodies, Monoclonal
biology.organism_classification
Urease
Virology
Infectious Diseases
medicine.anatomical_structure
Bacterial Vaccines
biology.protein
Molecular Medicine
Electrophoresis, Polyacrylamide Gel
Female
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....11ab380bd59fd0c141ea229274013592