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Poorly differentiated desmin-negative and vimentin-positive leiomyosarcoma of the stomach examined by the immunohistochemical and quick-freezing and deep-etching methods
- Source :
- Virchows Archiv. 432:377-383
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- A poorly differentiated leiomyosarcoma of the stomach in a 41-year-old woman is reported. The diagnosis was confirmed by the diffuse immunohistochemical reaction to HHF35, and the presence of focal density and caveolas in some of the tumour cells by conventional electron microscopy. Immunohistochemically, most tumour cells had an undifferentiated nature, in which negative immunostaining for desmin, alpha-smooth muscle actin, and type IV collagen, and positive immunostaining for vimentin were observed. By the quick-freezing and deep-etching (QF-DE) method, these tumour cells revealed the loss of bundled actin and myosin filaments, which constitute desmin associated structures (focal densities and dense patchy areas). Their cytoplasm had many mitochondria and other cell organelles. The intermediate filaments (IFs), which were determined to be vimentin by immunohistochemistry, were observed in the inter-organellar spaces, and connected with these cell organelles. Actin filaments formed a meshwork structure and were distributed mainly in subplasmalemmal regions. Although a basal lamina was not detected by conventional electron microscopy, basal lamina-like structures, an association between the extracellular matrices and the cell membrane, were observed. Using the QF-DE method, three dimensional ultrastructural alterations of the cytoskeleton and extracellular matrix of the leiomyosarcoma were observed.
- Subjects :
- Adult
Leiomyosarcoma
Pathology
medicine.medical_specialty
Vimentin
macromolecular substances
Desmin
Pathology and Forensic Medicine
Extracellular matrix
Stomach Neoplasms
Biomarkers, Tumor
medicine
Humans
Cytoskeleton
Intermediate filament
Molecular Biology
biology
Freeze Etching
Cell Biology
General Medicine
Immunohistochemistry
Actins
medicine.anatomical_structure
Cytoplasm
biology.protein
Female
Basal lamina
Immunostaining
Subjects
Details
- ISSN :
- 14322307 and 09456317
- Volume :
- 432
- Database :
- OpenAIRE
- Journal :
- Virchows Archiv
- Accession number :
- edsair.doi.dedup.....11b737b3061249769255425073e2bbba