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Ultraviolet and visible light spectrophotometric approach to blood typing: objective analysis by agglutination index
- Source :
- Transfusion. 39:1051-1059
- Publication Year :
- 1999
- Publisher :
- Wiley, 1999.
-
Abstract
- BACKGROUND: A new blood typing technology based on ultraviolet (UV) and visible light spectroscopy (UV/visible spectroscopy) has been developed. Blood groups and types are determined by quantifying reproducible changes in the UV and visible light spectra of blood in the presence of agglutinating antibodies. STUDY DESIGN AND METHODS: Samples of red cells in the presence and absence of agglutinating antibodies were examined by UV/visible spectroscopy. Blood groups and types were determined by comparing the optical density spectra obtained between 665 and 1000 nm. These comparisons generate numbers (agglutination index) ranging from 0 to 100, with smaller numbers corresponding to lack of agglutination and larger numbers corresponding to agglutination. RESULTS: The optical density of agglutinated blood is dramatically different from that of unagglutinated blood. The agglutination index derived from the relative slopes of the spectra is an objective indicator of agglutination strength. An agglutination index greater than 17 consistently and accurately established blood group- and type-specific agglutination. CONCLUSION: The method accurately predicted A, B, and O blood groups, and D type in over 275 samples. Scattering theory-based calculations of relative volumes of red cells before and after agglutination show a direct correlation with the agglutination index and provide the theoretical basis of the analysis. This quantitative technique is reproducible and has the potential for automation.
- Subjects :
- Hemagglutination
Ultraviolet Rays
Agglutination
Immunology
medicine.disease_cause
Models, Biological
Sensitivity and Specificity
Blood typing
Ultraviolet visible spectroscopy
Spectrophotometry
medicine
Humans
Scattering, Radiation
Immunology and Allergy
Spectroscopy
Chromatography
medicine.diagnostic_test
Chemistry
Reproducibility of Results
Hemagglutination Tests
Hematology
Blood Grouping and Crossmatching
Ultraviolet
Visible spectrum
Subjects
Details
- ISSN :
- 15372995 and 00411132
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Transfusion
- Accession number :
- edsair.doi.dedup.....11bb5b6c0c5f267a66a634b0b9540a55