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Elevated expression of monocyte chemoattractant protein 1 by vascular smooth muscle cells in hypercholesterolemic primates
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 89(15)
- Publication Year :
- 1992
-
Abstract
- Atherosclerosis is marked by an overt inflammatory infiltrate, with enhanced recruitment of monocytes/macrophages observed in both human and experimental atherosclerosis. We previously determined that monocyte chemoattractant protein 1 (MCP-1) accounts for virtually all of the chemotactic activity produced by vascular (aortic) smooth muscle cells in culture. We now report that arteries from a primate model of atherosclerosis with dietary-induced hypercholesterolemia exhibit increased levels of MCP-1 mRNA expression in vivo, whereas their normal counterparts demonstrate minimal MCP-1 expression. Furthermore, immunohistochemistry and in situ hybridization clearly indicate that the expression of MCP-1 protein and mRNA is in the smooth muscle cells of the medial layer of the artery and in monocyte-like and smooth muscle-like cells found in the overlying intimal lesion. These studies indicate that one of the responses to dietary hypercholesterolemia is the expression of MCP-1 by vascular smooth muscle cells. This expression, when augmented with other cellular and molecular factors, could significantly contribute to the recruitment of monocytes/macrophages to the vessel wall.
- Subjects :
- medicine.medical_specialty
Vascular smooth muscle
Hypercholesterolemia
In situ hybridization
Biology
Muscle, Smooth, Vascular
Pathogenesis
Lesion
Cholesterol, Dietary
Reference Values
Internal medicine
medicine
Animals
RNA, Messenger
Chemokine CCL2
Messenger RNA
Multidisciplinary
Chemotactic Factors
Vascular disease
Monocyte
Nucleic Acid Hybridization
medicine.disease
Blotting, Northern
Macaca fascicularis
medicine.anatomical_structure
Endocrinology
Carotid Arteries
Cholesterol
Immunology
Diet, Atherogenic
RNA
medicine.symptom
Artery
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 89
- Issue :
- 15
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....11c2129f4c74d0c4e10c7cd69272bf30