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The major-effect quantitative trait locus Fnl7.1 encodes a late embryogenesis abundant protein associated with fruit neck length in cucumber
- Source :
- Plant Biotechnology Journal
- Publication Year :
- 2019
-
Abstract
- Summary Fruit neck length (FNL) is an important quality trait in cucumber because it directly affects its market value. However, its genetic basis remains largely unknown. We identified a candidate gene for FNL in cucumber using a next‐generation sequencing‐based bulked segregant analysis in F2 populations, derived from a cross between Jin5‐508 (long necked) and YN (short necked). A quantitative trait locus (QTL) on chromosome 7, Fnl7.1, was identified through a genome‐wide comparison of single nucleotide polymorphisms between long and short FNL F2 pools, and it was confirmed by traditional QTL mapping in multiple environments. Fine genetic mapping, sequences alignment and gene expression analysis revealed that CsFnl7.1 was the most likely candidate Fnl7.1 locus, which encodes a late embryogenesis abundant protein. The increased expression of CsFnl7.1 in long‐necked Jin5‐508 may be attributed to mutations in the promoter region upstream of the gene body. The function of CsFnl7.1 in FNL control was confirmed by its overexpression in transgenic cucumbers. CsFnl7.1 regulates fruit neck development by modulating cell expansion. Probably, this is achieved through the direct protein–protein interactions between CsFnl7.1 and a dynamin‐related protein CsDRP6 and a germin‐like protein CsGLP1. Geographical distribution differences of the FNL phenotype were found among the different cucumber types. The East Asian and Eurasian cucumber accessions were highly enriched with the long‐necked and short‐necked phenotypes, respectively. A further phylogenetic analysis revealed that the Fnl7.1 locus might have originated from India. Thus, these data support that the CsFnl7.1 has an important role in increasing cucumber FNL.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Candidate gene
QTL
Quantitative Trait Loci
India
Locus (genetics)
Single-nucleotide polymorphism
Plant Science
Biology
Quantitative trait locus
01 natural sciences
03 medical and health sciences
Gene mapping
Gene expression
late embryogenesis abundant protein
fruit neck length
Gene
Phylogeny
Research Articles
Genetics
Cucumber
Bulked segregant analysis
030104 developmental biology
Phenotype
Fruit
fine genetic mapping
Cucumis sativus
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Research Article
Subjects
Details
- ISSN :
- 14677652
- Volume :
- 18
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Plant biotechnology journal
- Accession number :
- edsair.doi.dedup.....11cddeed8b2edf70bf96638a8770b132