Back to Search
Start Over
Tat-associated kinase (P-TEFb): a component of transcription preinitiation and elongation complexes
- Source :
- The Journal of biological chemistry. 274(11)
- Publication Year :
- 1999
-
Abstract
- Human immunodeficiency virus, type 1 (HIV-1) Tat protein activates transcription from the HIV-1 long terminal repeat. Tat interacts with TFIIH and Tat-associated kinase (a transcription elongation factor P-TEFb) and requires the carboxyl-terminal domain of the largest subunit of RNA polymerase II (pol II) for transactivation. We developed a stepwise RNA pol II walking approach and used Western blotting to determine the role of TFIIH and P-TEFb in HIV-1 transcription elongation. Our results demonstrate the new findings that P-TEFb is a component of the preinitiation complex and travels with the elongating RNA pol II, whereas TFIIH is released from the elongation complexes before the trans-activation responsive region RNA is synthesized. Our results suggest that TFIIH and P-TEFb are involved in the clearance of promoter-proximal pausing of RNA pol II on the HIV-1 long terminal repeat at different stages.
- Subjects :
- biology
Transcription, Genetic
Cell Biology
Protein Serine-Threonine Kinases
Peptide Elongation Factors
Biochemistry
Molecular biology
Transcription Factors, TFII
Transcription Factor TFIIH
Transcription preinitiation complex
Gene Products, tat
biology.protein
Humans
Transcription factor II F
Positive Transcriptional Elongation Factor B
Transcription factor II E
RNA Polymerase II
P-TEFb
Molecular Biology
RNA polymerase II holoenzyme
Transcription factor II B
Transcription factor II A
HeLa Cells
Transcription Factors
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 274
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....11e7ff6539b684f96bf65f0000c3e9c9