Back to Search
Start Over
Colonization of Beauveria bassiana 08F04 in root-zone soil and its biocontrol of cereal cyst nematode (Heterodera filipjevi)
- Source :
- PLoS ONE, Vol 15, Iss 5, p e0232770 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Cereal cyst nematodes cause serious yield losses of wheat in Hunaghuai winter wheat growing region in China. Beauveria bassiana 08F04 isolated from the surface of cysts is a promising biological control agent for cereal cyst nematodes. As the colonization capacity is a crucial criteria to assess biocontrol effectiveness for a microbial agent candidate, we aimed to label B. bassiana 08F04 for efficient monitoring of colonization in the soil. The binary pCAM-gfp plasmid containing sgfp and hph was integrated into B. bassiana 08F04 using the Agrobacterium tumefaciens-mediated transformation. The transformation caused a significant change in mycelial and conidial yields, and in extracellular chitinase activity in some transformants. The cultural filtrates of some transformants also decreased acetylcholinesterase activity and the survival of Heterodera filipjevi second-stage juveniles relative to the wild-type strain. One transformant (G10) had a growth rate and biocontrol efficacy similar to the wild-type strain, so it was used for a pilot study of B. bassiana colonization conducted over 13 weeks. Real-time PCR results and CFU counts revealed that the population of G10 increased quickly over the first 3 weeks, then decreased slowly over the following 4 weeks before stabilizing. In addition, the application of wild-type B. bassiana 08F04 and transformant G10 significantly reduced the number of H. filipjevi females in roots by 64.4% and 60.2%, respectively. The results of this study have practical applications for ecological, biological and functional studies of B. bassiana 08F04 and for bionematicide registration.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Agrobacteria
Population Dynamics
Culture
Biological pest control
Social Sciences
Artificial Gene Amplification and Extension
Plant Science
Bassiana
Polymerase Chain Reaction
Biochemistry
Plant Roots
01 natural sciences
Fluorescence Microscopy
Plant Microbiology
Sociology
Colonization
Soil Microbiology
Triticum
Mycelium
Microscopy
education.field_of_study
Multidisciplinary
Heterodera filipjevi
biology
Light Microscopy
Eukaryota
Proteases
Plants
Enzymes
Horticulture
Wheat
Medicine
Female
Research Article
Tylenchida
Science
Population
Beauveria bassiana
Research and Analysis Methods
Agrobacterium Tumefaciens
Microbiology
03 medical and health sciences
Transformation, Genetic
Animals
Grasses
Beauveria
Molecular Biology Techniques
Pest Control, Biological
education
Molecular Biology
Plant Diseases
Cereal cyst nematode
Population Biology
Bacteria
fungi
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
030104 developmental biology
Enzymology
Serine Proteases
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....122efa28b334ad188d1f6bb18201979e