Direct labeling of dna-adducts formed from carcinogenic diol-epoxides with a fluorescent reporter compound specific for the cis vic-diol group

The fluorescent reporter group, N-(5-FLUORESCEINYL),N′-(3-BORONATOPHENYL)THIOUREA (FABA)has been synthesized. This boronate group makes the reporter specific for cis vic-diols. The reporter group is bound to DNA-adducts formed from the reaction of calf-thymus DNA and benzanthracene trans-10,11-dihydrodiol,8,9-epoxide (anti), benzo(a)anthracene-trans-3,4-dihydrodiol-1,2-epoxide (anti) but is not bound to 3-methylcholanthrene-11,12 dihydro-epoxide. Femtomole quantities of adduct may be detected.