Back to Search
Start Over
Systemic lidocaine inhibits high-mobility group box 1 messenger ribonucleic acid expression and protein in BALB/c mice after closed fracture musculoskeletal injury
- Source :
- Saudi Journal of Anaesthesia, Vol 12, Iss 3, Pp 395-398 (2018), Saudi Journal of Anaesthesia
- Publication Year :
- 2018
- Publisher :
- Wolters Kluwer Medknow Publications, 2018.
-
Abstract
- Background: Severe musculoskeletal trauma can trigger an inflammatory response, and an excessive inflammatory response can lead to systemic inflammatory response syndrome and multiorgan failure. High-mobility group box 1 (HMGB1) is an early mediator pro-inflammatory cytokine in sterile injuries and a late cytokine mediator in infection and sepsis. Previous research has shown that administration of systemic lidocaine can inhibit HMGB1 expression in macrophages of septic rats. The aim of this study was to demonstrate the efficacy of systemic lidocaine to inhibit HMGB1 mRNA and protein in a BALB/c mouse model of sterile inflammation due to closed fracture musculoskeletal injury. Materials and Methods: Twenty adult male BALB/c mice were divided into lidocaine and control groups. The closed fracture musculoskeletal injury was performed by breaking the left thigh bone of the mice. Four hours after undergoing the closed fracture, the lidocaine group was treated with lidocaine intravenous (2 mg/kg). The same volume of distilled water was injected into the control group instead of lidocaine. HMGB1 mRNA expression was examined with real-time polymerase chain reaction, and HMGB1 protein level was determined with enzyme-linked immunosorbent assay. Results: The expression of HMGB1 mRNA and protein levels in mice that sustained inflammation due to a closed fracture musculoskeletal injury was significantly decreased in the lidocaine group (P < 0.00 and P < 0.00 for mRNA and protein, respectively). Conclusions: Intravenous administration of lidocaine effectively inhibited the inflammatory process in BALB/c mice that underwent closed fracture musculoskeletal injury by suppressing HMGB1 mRNA transcription and HMGB1 protein translation.
- Subjects :
- 0301 basic medicine
Lidocaine
medicine.medical_treatment
Inflammation
chemical and pharmacologic phenomena
Pharmacology
HMGB1
BALB/c
Sepsis
lcsh:RD78.3-87.3
03 medical and health sciences
Closed Fracture
0302 clinical medicine
medicine
Closed fracture
lidocaine
mRNA high-mobility group box 1
biology
business.industry
biology.organism_classification
medicine.disease
Systemic inflammatory response syndrome
030104 developmental biology
Anesthesiology and Pain Medicine
Cytokine
lcsh:Anesthesiology
biology.protein
Original Article
medicine.symptom
business
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- Language :
- English
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Anaesthesia
- Accession number :
- edsair.doi.dedup.....128145b7d3c2c6df8b79a60e2ece0145