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Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase

Authors :
Tobias Georgi
Volker F. Wendisch
Doris Rittmann
Source :
Metabolic Engineering. 7:291-301
Publication Year :
2005
Publisher :
Elsevier BV, 2005.

Abstract

In the biotechnological production Of L-lysine and L-glutamate by Corynebacterium glutamicum media based on glucose, fructose or sucrose are typically used. Glutamate production by C glutamicum ATCC13032 was very similar on glucose, fructose, glucose plus fructose and sucrose. In contrast, lysine production of genetically defined C glutamicum strains was significantly higher on glucose than on the other carbon sources. To test whether malic enzyme or fructose-1,6-bisphosphatase might limit growth and lysine on fructose, glucose plus fructose or sucrose, strains overexpressing either malE which encodes the NADPH-dependent malic enzyme or the fructose-1,6-bisphosphatase gene fbp were generated. Overexpression of malE did not improve lysine production on any of the tested carbon sources. Upon overexpression of fbp lysine yields on glucose and/or fructose were unchanged, but the lysine yield on sucrose increased twofold. Thus, fructose-1,6-bisphosphatase was identified as a limiting factor for lysine production by C glutamicum with sucrose as the carbon source. (c) 2005 Elsevier Inc. All rights reserved.

Details

ISSN :
10967176
Volume :
7
Database :
OpenAIRE
Journal :
Metabolic Engineering
Accession number :
edsair.doi.dedup.....12ac37bbc86d774b9467ed838cae20b1
Full Text :
https://doi.org/10.1016/j.ymben.2005.05.001