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Quantification of emerging micropollutants in an amphipod crustacean by nanoliquid chromatography coupled to mass spectrometry using multiple reaction monitoring cubed mode

Quantification of emerging micropollutants in an amphipod crustacean by nanoliquid chromatography coupled to mass spectrometry using multiple reaction monitoring cubed mode

Authors :
Alexandra Berlioz-Barbier
Martin Sordet
Emmanuelle Vulliet
Audrey Buleté
Jeanne Garric
TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé
Institut des Sciences Analytiques (ISA)
Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-École normale supérieure - Lyon (ENS Lyon)
Milieux aquatiques, écologie et pollutions (UR MALY)
Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)
Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Source :
Journal of Chromatography A, Journal of Chromatography A, Elsevier, 2016, 1456, pp.217-225. ⟨10.1016/j.chroma.2016.06.022⟩, Journal of Chromatography A, Elsevier, 2016, 1456 (1456), pp.217-225. ⟨10.1016/j.chroma.2016.06.022⟩
Publication Year :
2016
Publisher :
Elsevier BV, 2016.

Abstract

International audience; An innovative analytical method has been developed to quantify the bioaccumulation in an amphipod crustacean (Gammarus fossarum) of three micropollutants regarded as anthropic-pollution markers: carbamazepine, oxazepam, and testosterone. A liquid-liquid extraction assisted by salts, known as QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) was miniaturised and optimised, so it could be adapted to the low mass samples (approximatively 5 mg dry weight). For this same reason and in order to obtain good sensitivity, ultra-trace analyses were carried out by means of nanoliquid chromatography. A pre-concentration system by on-column trapping was optimised to increase the injection volume. In order to improve both sensitivity and selectivity, the multiple reaction monitoring cubed mode analyses (MRM3) were carried out, validated and compared to the classic MRM. To the best of our knowledge, this is the first time that MRM3 is coupled to nanoliquid chromatography for the analysis and detection of organic micropollutants

Details

ISSN :
00219673 and 18733778
Volume :
1456
Database :
OpenAIRE
Journal :
Journal of Chromatography A
Accession number :
edsair.doi.dedup.....12ad18fe27aca3242cfdfaf87681189e
Full Text :
https://doi.org/10.1016/j.chroma.2016.06.022