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Multiplex PCR Method for Use in Real-Time PCR for Identification of Fish Fillets from Grouper (Epinephelus and Mycteroperca Species) and Common Substitute Species
- Source :
- Journal of Agricultural and Food Chemistry. 53:2039-2045
- Publication Year :
- 2005
- Publisher :
- American Chemical Society (ACS), 2005.
-
Abstract
- Mitochondrial 16S rRNA sequences from morphological validated grouper (Epinephelus aeneus, E. caninus, E. costae, and E. marginatus; Mycteroperca fusca and M. rubra), Nile perch (Lates niloticus), and wreck fish (Polyprion americanus) were used to develop an analytical system for group diagnosis based on two alternative Polymerase Chain Reaction (PCR) approaches. The first includes conventional multiplex PCR in which electrophoretic migration of different sizes of bands allowed identification of the fish species. The second approach, involving real-time PCR, produced a single amplicon from each species that showed different Tm values allowing the fish groups to be directly identified. Real-time PCR allows the quick differential diagnosis of the three groups of species and high-throughput screening of multiple samples. Neither PCR system cross-reacted with DNA samples from 41 common marketed fish species, thus conforming to standards for species validation. The use of these two PCR-based methods makes it now possible to discriminate grouper from substitute fish species.
- Subjects :
- biology
Fishes
Zoology
Fish fillet
DNA
General Chemistry
Epinephelus
Amplicon
biology.organism_classification
Polymerase Chain Reaction
Perciformes
Fishery
Mycteroperca fusca
Fish Products
Epinephelus aeneus
Multiplex polymerase chain reaction
Animals
Grouper
General Agricultural and Biological Sciences
Mycteroperca
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....12b8f1a8c34a3b02e002a0288f3d6028