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Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis

Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis

Authors :
Carol Chuang
Jing Pan
Kimberly N. Weiderhold
Arpaporn Deeraksa
Li Yuan Yu-Lee
Maria Fadri-Moskwik
Sue Hwa Lin
Michiya Nishino
Source :
PLoS ONE, Vol 11, Iss 4, p e0153455 (2016), PLoS ONE
Publication Year :
2016
Publisher :
Public Library of Science (PLoS), 2016.

Abstract

Nuclear distribution protein C (NudC) is a mitotic regulator that plays a role in cytokinesis. However, how NudC is regulated during cytokinesis remains unclear. Here, we show that NudC is phosphorylated by Aurora B, a kinase critical for cell abscission. NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. Inhibition of Aurora B by ZM447439 reduced NudC phosphorylation, suggesting that NudC is an Aurora B substrate in vivo. We identified T40 on NudC as an Aurora B phosphorylation site. NudC depletion resulted in cytokinesis failure with a dramatic elongation of the intercellular bridge between daughter cells, sustained Aurora B activity at the midbody, and reduced cell abscission. These cytokinetic defects can be rescued by the ectopic expression of wild-type NudC. Reconstitution with T40A phospho-defective NudC was found to rescue the cytokinesis defect. In contrast, reconstitution with the T40D phospho-mimetic NudC was inefficient in supporting the completion of cytokinesis. These results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis.

Details

Language :
English
ISSN :
19326203
Volume :
11
Issue :
4
Database :
OpenAIRE
Journal :
PLoS ONE
Accession number :
edsair.doi.dedup.....130820ee5ae2c7cadea4e392256552db