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Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis
Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis
- Source :
- PLoS ONE, Vol 11, Iss 4, p e0153455 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Nuclear distribution protein C (NudC) is a mitotic regulator that plays a role in cytokinesis. However, how NudC is regulated during cytokinesis remains unclear. Here, we show that NudC is phosphorylated by Aurora B, a kinase critical for cell abscission. NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. Inhibition of Aurora B by ZM447439 reduced NudC phosphorylation, suggesting that NudC is an Aurora B substrate in vivo. We identified T40 on NudC as an Aurora B phosphorylation site. NudC depletion resulted in cytokinesis failure with a dramatic elongation of the intercellular bridge between daughter cells, sustained Aurora B activity at the midbody, and reduced cell abscission. These cytokinetic defects can be rescued by the ectopic expression of wild-type NudC. Reconstitution with T40A phospho-defective NudC was found to rescue the cytokinesis defect. In contrast, reconstitution with the T40D phospho-mimetic NudC was inefficient in supporting the completion of cytokinesis. These results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis.
- Subjects :
- 0301 basic medicine
Cell division
Cultured tumor cells
lcsh:Medicine
Cell Cycle Proteins
Biochemistry
Microtubules
chemistry.chemical_compound
Aurora Kinase B
Small interfering RNAs
Cell Cycle and Cell Division
Phosphorylation
Post-Translational Modification
lcsh:Science
Cytoskeleton
Staining
Multidisciplinary
Chromosome Biology
Nuclear Proteins
Cell Staining
Precipitation Techniques
3. Good health
ZM447439
Cell biology
Nucleic acids
Midbody
Cell Processes
embryonic structures
Cell lines
Cellular Structures and Organelles
biological phenomena, cell phenomena, and immunity
Biological cultures
Research Article
Aurora B kinase
Mitosis
macromolecular substances
Biology
Research and Analysis Methods
03 medical and health sciences
Microtubule
Genetics
Humans
Immunoprecipitation
HeLa cells
Non-coding RNA
Cytokinesis
lcsh:R
Biology and Life Sciences
Proteins
Cell Biology
Cell cultures
Molecular biology
Gene regulation
enzymes and coenzymes (carbohydrates)
030104 developmental biology
chemistry
Specimen Preparation and Treatment
RNA
lcsh:Q
Gene expression
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....130820ee5ae2c7cadea4e392256552db