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Inhibitory effects of long-chain alkyltrimethylammonium ions on aggregation of bovine platelets and the relation of their effects to Ca2+ mobilization
- Source :
- Biochimica et Biophysica Acta (BBA) - Biomembranes. 905:75-80
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- The inhibitory efefcts of alkyltrimethylammonium ions on ADP-and thrombin-induced aggregation of bovine platelets were investigated. The ammonium cations inhibited the two aggregation reactions to similar extents. The relationship between their inhibitory efects on ADP-induced aggregation and their alkyl chain lengths from C 8 to C 18 was investigated. Results showed that the inhibitory effects of ammonium cations increased with increase of their alkyl chain lengths up to C 16 , and that the increase was linear with chain lengths of up to C 14 . This linear relation and slope of the linear regression line suggested that the inhibitory effects of the ammonium cations depended on their partitioning into the membrane. However, unlike long-chain unsaturated fatty acids, they did not affect the membrane fluidity of the platelets, Fluorescence analysis of fura-2 loaded platelets revealed that, in the concentration range where the alkyltrimethylammonium ions inhibited aggregation, they inhibited agonist-induced increase in cytosolic Ca 2+ both in the presence and absence of extracellular Ca 2+ . These results suggest that inhibition of platelet aggregation by alkyltrimethylammonium ionsis mainly due to their inhibition of increase in cytoplasmic Ca 2+ by inhibition of both intracellular Ca + mobilization and Ca 2+ uptake.
- Subjects :
- Blood Platelets
Platelet Aggregation
Membrane Fluidity
Biophysics
chemistry.chemical_element
Calcium
Inhibitory postsynaptic potential
Biochemistry
chemistry.chemical_compound
Cytosol
Membrane fluidity
Extracellular
Animals
Platelet
Ammonium
Alkyl
chemistry.chemical_classification
Thrombin
Biological Transport
Cell Biology
Adenosine Diphosphate
Molecular Weight
Quaternary Ammonium Compounds
Membrane
chemistry
Cattle
Platelet Aggregation Inhibitors
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 905
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Accession number :
- edsair.doi.dedup.....13406a7c00b71bf168c649400262a5b5