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Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area
- Source :
- Malaria Journal, Yan, J; Li, N; Wei, X; Li, P; Zhao, Z; Wang, L; et al.(2013). Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area. Malaria Journal, 12(1). doi: 10.1186/1475-2875-12-73. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/4zc636f3, Yan, Juan; Li, Nana; Wei, Xu; Li, Peipei; Zhao, Zhenjun; Wang, Lili; et al.(2013). Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area. Malaria Journal, 12(1), 73. doi: http://dx.doi.org/10.1186/1475-2875-12-73. Retrieved from: http://www.escholarship.org/uc/item/62r2789w
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Background Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area. Methods A total 606 febrile patients in the China-Myanmar border were recruited to this study and were diagnosed for malaria infections by microscopy, two RDTs tests (Pf/Pan device, and Pv/Pf device) and nested PCR. Results Malaria parasites were found in 143 patients by microscopy, of which 51, 73, and 19 were Plasmodium falciparum, Plasmodium vivax and P. falciparum/P. vivax mixed infections, respectively. Compared to microscopy, the sensitivity of the Pf/Pan device was 88.6% for P. falciparum and 69.9% for P. vivax with the specificity of 90.4%. For a subset of 350 patients, the sensitivity of the Pf/Pan device and Pv/Pf device for detection of P. falciparum was 87.5% and 91.7%, respectively; and for detection of P. vivax was 72.0% and 73.8%, respectively. The specificity of the Pf/Pan device and Pv/Pf device was 94.3% and 96.5%, respectively. Nested PCR detected malaria parasites in 174 of 606 samples, of which 67, 79, two and 26 were P. falciparum, P. vivax, P. ovale and P. falciparum/P. vivax mixed infections, respectively. Compared to nested PCR, all other methods had sensitivity below 80%, suggesting that a significant number of cases were missed. Conclusions Compared to PCR, both microscopy and RDTs had lower sensitivities. RDTs had similar performance to microscopy for P. falciparum diagnosis, but performed worse for P. vivax diagnosis. Other RDT products should be selected with higher sensitivity (and good specificity) for both P. falciparum and P. vivax diagnosis.
- Subjects :
- Male
Plasmodium ovale
Plasmodium vivax
Myanmar
Sensitivity
0302 clinical medicine
030212 general & internal medicine
Child
Aged, 80 and over
Microscopy
Diagnostic test
Middle Aged
3. Good health
PCR
Infectious Diseases
Child, Preschool
Specificity
Female
Adult
China
medicine.medical_specialty
Adolescent
Plasmodium falciparum
030231 tropical medicine
Biology
Sensitivity and Specificity
Young Adult
03 medical and health sciences
parasitic diseases
medicine
Humans
Aged
Malaria diagnosis
Clinical Laboratory Techniques
Diagnostic Tests, Routine
Research
Infant
biology.organism_classification
medicine.disease
Virology
Rapid diagnostic tests (RDTs)
Malaria
Parasitology
Immunology
Tropical medicine
Nested polymerase chain reaction
Subjects
Details
- ISSN :
- 14752875
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Malaria Journal
- Accession number :
- edsair.doi.dedup.....137ec26b5f6069139fd85964b771239b