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Vascular Endothelial Growth Factor Up-regulation in Human Amniotic Fluid Stem Cell Enhances Nephroprotection After Ischemia-Reperfusion Injury in the Rat
- Source :
- Critical care medicine. 45(1)
- Publication Year :
- 2016
-
Abstract
- OBJECTIVE: To evaluate if the up-regulation of vascular endothelial growth factor strengthens the protective effect of amniotic fluid stem cells in a renal ischemia-reperfusion injury model. DESIGN: Randomized animal study. SETTINGS: University research laboratory. SUBJECTS: A total of 40 males 12-week-old Wistar rats were subjected to ischemia-reperfusion and assigned to four groups: amniotic fluid stem cells, vascular endothelial growth factor-amniotic fluid stem cells in two different doses, and vehicle. Ten animals were used as sham-controls. INTERVENTION: Six hours after induction of renal ischemia-reperfusion injury, amniotic fluid stem cells, vascular endothelial growth factor-amniotic fluid stem cells in two different doses, or vehicle were injected intraarterially. MEASUREMENTS AND MAIN RESULTS: Analyses were performed at 24 hours, 48 hours, and 2 months after treatment. Outcome measures included serum creatinine, urine microprotenuira, and immunohistomorphometric analyses. Vascular endothelial growth factor-amniotic fluid stem cells induced a significantly higher nephroprotection than amniotic fluid stem cells. This effect was mediated mainly by immunomodulation, which led to lower macrophage infiltration and higher presence of regulatory T cell after ischemia-reperfusion injury. At medium term, it inhibited the progression toward chronic kidney disease. Vascular endothelial growth factor-amniotic fluid stem cells can worsen the ischemia-reperfusion injury when delivered in a high dose. CONCLUSIONS: Up-regulation of vascular endothelial growth factor enhances the therapeutic effect of human amniotic fluid stem cells in rats with renal ischemia-reperfusion injury, mainly by mitogenic, angiogenic, and anti-inflammatory mechanisms. ispartof: Crit Care Med vol:45 issue:1 pages:e86-e96 ispartof: location:United States status: published
- Subjects :
- 0301 basic medicine
Vascular Endothelial Growth Factor A
Amniotic fluid
Regulatory T cell
Ischemia
Pharmacology
Critical Care and Intensive Care Medicine
T-Lymphocytes, Regulatory
03 medical and health sciences
chemistry.chemical_compound
Medicine
Animals
Rats, Wistar
Renal Insufficiency, Chronic
business.industry
Macrophages
Stem Cells
Amniotic stem cells
Acute Kidney Injury
medicine.disease
Amniotic Fluid
Up-Regulation
Vascular endothelial growth factor
030104 developmental biology
medicine.anatomical_structure
chemistry
Injections, Intra-Arterial
Reperfusion Injury
Immunology
Disease Progression
Stem cell
business
Reperfusion injury
Kidney disease
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 15300293
- Volume :
- 45
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Critical care medicine
- Accession number :
- edsair.doi.dedup.....13a4484b481ffcc57e129d685b98162c