Back to Search
Start Over
A 64 kDa protein is a candidate for a thyrotropin-releasing hormone receptor in prolactin-producing rat pituitary tumor cells (GH4C1 cells)
- Source :
- Biochemical and biophysical research communications. 157(3)
- Publication Year :
- 1988
-
Abstract
- A thyrotropin-releasing hormone (TRH) binding protein of 64 kDa has been identified by covalently crosslinking [3H]TRH to GH4C1 cells by ultraviolet illumination. The crosslinkage of [3H]TRH is UV-dose dependent and is inhibited by an excess of unlabeled TRH. A 64 kDa protein is also detected on immunoblots using an antiserum raised against GH4C1 cell surface epitopes. In a closely related cell line (GH12C1) which does not bind [3H]TRH, the 64 kDa protein cannot be demonstrated by [3H]TRH crosslinking nor by immunoblotting. These findings indicate that the 64 kDa protein is a candidate for a TRH-receptor protein in GH4C1 cells.
- Subjects :
- endocrine system
endocrine system diseases
G protein
Photochemistry
Ultraviolet Rays
Immunoblotting
Biophysics
Biology
Peptide hormone
Biochemistry
Epitope
Thyrotropin-releasing hormone receptor
Tumor Cells, Cultured
Animals
Pituitary Neoplasms
Receptor
Molecular Biology
Thyrotropin-Releasing Hormone
Binding protein
Receptors, Thyrotropin-Releasing Hormone
Cell Membrane
Affinity Labels
Cell Biology
Prolactin
Rats
Receptors, Neurotransmitter
Molecular Weight
Cell culture
Electrophoresis, Polyacrylamide Gel
hormones, hormone substitutes, and hormone antagonists
Endocrine gland
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 157
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....13af4591e4b4a65ded3559c8b90e968d