Back to Search
Start Over
Pituitary transcription factor-1 induces transient differentiation of adult hepatic stem cells into prolactin-producing cells in vivo
- Source :
- Molecular endocrinology (Baltimore, Md.). 19(4)
- Publication Year :
- 2005
-
Abstract
- A subset of transcription factors function as pivotal regulators of cell differentiation pathways. Pituitary transcription factor-1 (Pit-1) is a tissue-specific homeodomain protein that specifies the development of pituitary somatotropes and lactotropes. In this study, adenovirus was used to deliver rat Pit-1 to mouse liver. Pit-1 expression was detected in the majority (50–80%) of hepatocyte nuclei after tail vein injection (2 × 109 plaque forming units). Pit-1 activated hepatic expression of the endogenous prolactin (PRL), GH, and TSHβ genes along with several other markers of lactotrope progenitor cells. Focal clusters (0.2–0.5% of liver cells per tissue section) of periportal cells were positive for PRL by immunofluorescent staining. The PRL-producing cells also expressed proliferating cell nuclear antigen as well as the hepatic stem cell markers (c-Kit, Thy1, and cytokeratin 14). These data indicate that Pit-1 induces the transient differentiation of hepatic progenitor cells into PRL-producing cells, providing additional evidence that transcription factors can specify the differentiation pathway of adult stem cells.
- Subjects :
- Male
medicine.medical_specialty
Liver cytology
Cellular differentiation
Gene Expression
Biology
Adenoviridae
Prolactin cell
Mice
Endocrinology
Internal medicine
Proliferating Cell Nuclear Antigen
medicine
Animals
RNA, Messenger
Progenitor cell
Molecular Biology
Cell potency
Stem Cells
Cell Differentiation
General Medicine
Cell biology
Prolactin
Rats
Endothelial stem cell
DNA-Binding Proteins
Pituitary Hormones
Liver
Hepatocytes
Female
Stem cell
Transcription Factor Pit-1
Adult stem cell
Transcription Factors
Subjects
Details
- ISSN :
- 08888809
- Volume :
- 19
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular endocrinology (Baltimore, Md.)
- Accession number :
- edsair.doi.dedup.....1401dee62febd054f494b2b617ab041d