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Changes in expression of interferon-stimulated genes and ubiquitin activating enzyme E1-like in ovine thymus during early pregnancy
- Source :
- Animal Reproduction, Volume: 17, Issue: 2, Article number: e20190134, Published: 08 JUL 2020, Animal Reproduction
- Publication Year :
- 2020
- Publisher :
- FapUNIFESP (SciELO), 2020.
-
Abstract
- As the main signal for the maternal recognition in ruminants, interferon-tau (IFNT) stimulates expression of interferon-stimulated genes (ISGs) in uterus and many extrauterine tissues. However, it is unclear that early pregnancy induces expression of signal transducer and activator of transcription 1 (STAT1), myxovirusresistance 1 (Mx1), interferon-gamma-inducible protein 10 (IP-10) and ubiquitin activating enzyme E1-like protein (UBE1L) in maternal thymus. In this study, ovine thymuses were sampled on day 16 of the estrous cycle and on days 13, 16 and 25 of gestation, and the expression of STAT1, Mx1, IP-10 and UBE1L was detected by real-time quantitative PCR, Western blot and immunohistochemistry. The results revealed that the expression of STAT1 and IP-10 reached peaks on day 16 of pregnancy, and expression of Mx1 was enhanced on day 25 of pregnancy, and STAT1 protein was located in the epithelial reticular cells, capillaries and thymic corpuscles. However, expression of UBE1L was declined during early pregnancy. In conclusion, early pregnancy influences expression of STAT1, Mx1, IP-10 and UBE1L in maternal thymus, which may participate in regulation of maternal immune tolerance during early pregnancy in sheep.
- Subjects :
- sheep
Ubiquitin-activating enzyme
ubiquitin activating enzyme E1-like
myxovirusresistance 1
Immune tolerance
Andrology
03 medical and health sciences
0302 clinical medicine
Western blot
interferon-gamma-inducible protein 10
medicine
STAT1
Estrous cycle
Pregnancy
030219 obstetrics & reproductive medicine
General Veterinary
medicine.diagnostic_test
biology
0402 animal and dairy science
04 agricultural and veterinary sciences
medicine.disease
040201 dairy & animal science
Real-time polymerase chain reaction
signal transducer and activator of transcription 1
biology.protein
STAT protein
Original Article
Animal Science and Zoology
Subjects
Details
- ISSN :
- 19843143 and 18069614
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Animal Reproduction
- Accession number :
- edsair.doi.dedup.....141ea31c55b70cf0d7c296b53bf611c9