Targets of protein carbonylation in spontaneously hypertensive obese Koletsky rats and healthy Wistar counterparts: A potential role on metabolic disorders

14 páginas, 6 figuras, 2 tablas, 1 apéndice
The study innovatively pinpoints target proteins of carbonylation, a key PTM induced by oxidative stress, in the SHROB (genetically obese spontaneously hypertensive) rat model of metabolic syndrome (MetS). Protein carbonylation was assessed by a fluorescence-labeling proteomics approach, and complemented with biometric and biochemical markers of MetS. SHROB and healthy Wistar rats were fed two diets, soybean and linseed oil supplementations, in order to distinguish intrinsic carbonylation of SHROB animals from diet-modulated carbonylation unrelated to MetS. First exploratory data showed similar carbonylation patterns and metabolic conditions in SHROB rats fed soybean and linseed, but different from Wistar animals. A total of 18 carbonylated spots in liver, and 12 in skeletal tissue, related to pathways of lipid (29.6%), carbohydrate (25.9%) and amino acid (18.5%) metabolisms, were identified. In particular, SHROB animals present higher carbonylation in four liver proteins belonging to lipid metabolism, redox regulation and chaperone activity (ALDH2, PDI, PDIA3, PECR), and in the skeletal muscle ALDOA that is involved in muscle dysfunction. Conversely, SHROB rats display lower carbonylation in liver albumin, AKR1C9, ADH1 and catalase. This investigation provides a novel perspective of carbonylation in the context of metabolic disorders, and may be a starting point to characterize new redox pathways exacerbating MetS
This investigation has been supported by the Spanish Ministry of Science and Innovation (Grant AGL2009-12374-C03-01, -02 and -03). The Spanish Ministry of Science and Innovation is gratefully acknowledged for the doctoral fellowship to L.M. L.M. also thanks the USC (Spain) for its doctoral program. Xunta de Galicia and European Social Fund are thankfully recognized by the financial support of the postdoctoral “Isidro Parga Pondal” contract to M.P. J.P.-J. thanks the Spanish Ministry of Science and Innovation and the Instituto de Salud Carlos III for granting her a Sara Borrell postdoctoral contract (CD09/00068)