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Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells
- Source :
- BioMed Research International, BioMed Research International, Vol 2020 (2020)
- Publication Year :
- 2020
- Publisher :
- Hindawi, 2020.
-
Abstract
- Recent comparisons between plant and animal viruses reveal many common principles that underlie how all viruses express their genetic material, amplify their genomes, and link virion assembly with replication. Cauliflower mosaic virus (CaMV) is not infectious for human beings. Here, we show that CaMV transactivator/viroplasmin protein (TAV) shares sequence similarity with and behaves like the human ribonuclease H1 (RNase H1) in reducing DNA/RNA hybrids detected with S9.6 antibody in HEK293T cells. We showed that TAV is clearly expressed in the cytosol and in the nuclei of transiently transfected human cells, similar to its distribution in plants. TAV also showed remarkable cytotoxic effects in U251 human glioma cells in vitro. These characteristics pave the way for future analysis on the use of the plant virus protein TAV, as an alternative to human RNAse H1 during gene therapy in human cells.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Article Subject
RNase P
Ribonuclease H
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Viral Proteins
Caulimovirus
Plant virus
Cell Line, Tumor
Ribonuclease H1
Cytotoxic T cell
Humans
Ribonuclease
General Immunology and Microbiology
biology
Cytotoxins
fungi
RNA
food and beverages
General Medicine
Transfection
Glioma
Glioma Cells
biology.organism_classification
Virology
030104 developmental biology
HEK293 Cells
Virion assembly
biology.protein
Medicine
Cauliflower mosaic virus
Cauliflower Mosaic Viru
010606 plant biology & botany
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2020
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....14400fe497b27ebddff88b541c543a26