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Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells
- Source :
- Materials, Materials, Vol 10, Iss 10, p 1168 (2017), Materials; Volume 10; Issue 10; Pages: 1168
- Publication Year :
- 2017
- Publisher :
- MDPI, 2017.
-
Abstract
- Periodontitis is a widespread dental disease affecting 10 to 15% of worldwide adult population, yet the current treatments are far from satisfactory. The human periodontal ligament stem cell is a promising potential seed cell population type in cell-based therapy and tissue regeneration, which require appropriate scaffold to provide a mimic extracellular matrix. Zein, a native protein derived from corn, has an excellent biodegradability, and therefore becomes a hotspot on research and application in the field of biomaterials. However, the high hydrophobicity of zein is unfavorable for cell adhesion and thus greatly limits its use. In this study, we fabricate co-electrospun zein/gelatin fiber scaffolds in order to take full advantages of the two natural materials and electrospun fiber structure. Zein and gelatin in four groups of different mass ratios (100:00, 100:20, 100:34, 100:50), and dissolved the mixtures in 1,1,1,3,3,3-hexafluoro-2-propanol, then produced membranes by electrospinning. The results showed that the scaffolds were smooth and homogeneous, as shown in scanning electron micrographs. The diameter of hybrid fibers was increased from 69 ± 22 nm to 950 ± 356 nm, with the proportion of gelatin increase. The cell affinity of zein/gelatin nanofibers was evaluated by using human periodontal ligament stem cells. The data showed that hydrophilicity and cytocompatibility of zein nanofibers were improved by blended gelatin. Taken together, our results indicated that the zein/gelatin co-electrospun fibers had sufficient mechanical properties, satisfied cytocompatibility, and can be utilized as biological scaffolds in the field of tissue regeneration.
- Subjects :
- 0301 basic medicine
cytocompatibility
Scaffold
Materials science
food.ingredient
Periodontal ligament stem cells
Population
02 engineering and technology
Gelatin
lcsh:Technology
Article
gelatin
03 medical and health sciences
food
zein
electrospun
human periodontal ligament stem cells
tissue engineering
Tissue engineering
Periodontal fiber
General Materials Science
education
lcsh:Microscopy
lcsh:QC120-168.85
education.field_of_study
lcsh:QH201-278.5
lcsh:T
food and beverages
021001 nanoscience & nanotechnology
Electrospinning
030104 developmental biology
lcsh:TA1-2040
Nanofiber
lcsh:Descriptive and experimental mechanics
lcsh:Electrical engineering. Electronics. Nuclear engineering
0210 nano-technology
lcsh:Engineering (General). Civil engineering (General)
lcsh:TK1-9971
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 19961944
- Volume :
- 10
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Materials
- Accession number :
- edsair.doi.dedup.....14527c38a76ef0eefb538839afc6aae7