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Pharmacological Treatment with Annexin A1 Reduces Atherosclerotic Plaque Burden in LDLR-/- Mice on Western Type Diet
- Source :
- PLoS ONE, Vol 10, Iss 6, p e0130484 (2015), PLoS ONE, PLOS ONE, 10(6):e0130484. Public Library of Science
- Publication Year :
- 2015
- Publisher :
- Public Library of Science, 2015.
-
Abstract
- OBJECTIVE: To investigate therapeutic effects of annexin A1 (anxA1) on atherogenesis in LDLR-/- mice. METHODS: Human recombinant annexin A1 (hr-anxA1) was produced by a prokaryotic expression system, purified and analysed on phosphatidylserine (PS) binding and formyl peptide receptor (FPR) activation. Biodistribution of 99mTechnetium-hr-anxA1 was determined in C57Bl/6J mice. 12 Weeks old LDLR-/- mice were fed a Western Type Diet (WTD) during 6 weeks (Group I) or 12 weeks (Group P). Mice received hr-anxA1 (1 mg/kg) or vehicle by intraperitoneal injection 3 times per week for a period of 6 weeks starting at start of WTD (Group I) or 6 weeks after start of WTD (Group P). Total aortic plaque burden and phenotype were analyzed using immunohistochemistry. RESULTS: Hr-anxA1 bound PS in Ca2+-dependent manner and activated FPR2/ALX. It inhibited rolling and adherence of neutrophils but not monocytes on activated endothelial cells. Half lives of circulating 99mTc-hr-anxA1 were
- Subjects :
- genetic structures
SURFACE-EXPRESSED PHOSPHATIDYLSERINE
lcsh:Medicine
Pharmacology
Mice
Medicine
A5
Receptor
lcsh:Science
IN-VIVO
NEUTROPHILS
Annexin A1
Mice, Knockout
Multidisciplinary
Drug Administration Routes
Plaque, Atherosclerotic
Recombinant Proteins
medicine.anatomical_structure
Disease Progression
lipids (amino acids, peptides, and proteins)
medicine.symptom
Research Article
endocrine system
Endothelium
Phagocytosis
Bone Marrow Cells
Inflammation
Immunophenotyping
MECHANISMS
PHAGOCYTOSIS
INFLAMMATION
In vivo
Animals
Humans
cardiovascular diseases
Blood Cells
FORMYL PEPTIDE RECEPTOR
business.industry
CLEAVAGE
Macrophages
Therapeutic effect
lcsh:R
nutritional and metabolic diseases
Disease Models, Animal
Receptors, LDL
Diet, Western
ENDOTHELIUM
Immunology
LDL receptor
lcsh:Q
business
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....147b7d20050c32be274b6f11b2f0d508