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Genetic diversity of Plasmodium falciparum histidine-rich protein 2 in the China-Myanmar border area
- Source :
- Li, Peipei; Xing, Hua; Zhao, Zhenjun; Yang, Zhaoqing; Cao, Yaming; Li, Wenli; et al.(2015). Genetic diversity of Plasmodium falciparum histidine-rich protein 2 in the ChinaâMyanmar border area. Acta Tropica, 152, 26-31. doi: 10.1016/j.actatropica.2015.08.003. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/7mm619fx, Li, P; Xing, H; Zhao, Z; Yang, Z; Cao, Y; Li, W; et al.(2015). Genetic diversity of Plasmodium falciparum histidine-rich protein 2 in the China-Myanmar border area. Acta Tropica, 152, 26-31. doi: 10.1016/j.actatropica.2015.08.003. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/8d75t7mr
- Publication Year :
- 2015
-
Abstract
- © 2015 Elsevier B.V. Deletion of the Plasmodium falciparum histidine-rich protein 2 (pfhrp2) gene may affect the performance of PfHRP2-based rapid diagnostic tests (RDTs). Here we investigated the genetic diversity of the pfhrp2 gene in clinical parasite isolates collected in recent years from the China-Myanmar border area. Deletion of pfhrp2 has been identified in 4 out of 97 parasite isolates. Sequencing of the pfhrp2 exon2 from 67 isolates revealed a high level of genetic diversity in pfhrp2, which is reflected in the presence of many repeat types and their variants, as well as variable copy numbers and different arrangements of these repeats in parasite isolates. In addition, we observed pfhrp3 deletion in three of the four parasites harboring pfhrp2 deletion, suggesting of double deletions of both genes in these three isolates. Analysis of two cases, which were P. falciparum-positive by microscopy and PCR but failed by two PfHRP2-based RDTs, did not find pfhrp2 deletion. Further correlational studies of pfhrp2 polymorphisms with detection sensitivity are needed to identify factors influencing the performance of RDTs in malaria-endemic areas.
- Subjects :
- China
Veterinary (miscellaneous)
Plasmodium falciparum
Protozoan Proteins
Antigens, Protozoan
Myanmar
Biology
Polymerase Chain Reaction
Article
law.invention
law
Genetic variation
parasitic diseases
medicine
Medicine and Health Sciences
Parasite hosting
Humans
Malaria, Falciparum
Gene
Histidine
Polymerase chain reaction
Sequence Deletion
Genetics
Genetic diversity
Polymorphism, Genetic
Diagnostic Tests, Routine
Genetic Variation
Proteins
medicine.disease
biology.organism_classification
Infectious Diseases
Insect Science
Parasitology
Malaria
Subjects
Details
- ISSN :
- 18736254
- Volume :
- 152
- Database :
- OpenAIRE
- Journal :
- Acta tropica
- Accession number :
- edsair.doi.dedup.....1482d6c3ffde28fe93d344d143d1a312