MOESM1 of Suppression of nicotinamide phosphoribosyltransferase expression by miR-154 reduces the viability of breast cancer cells and increases their susceptibility to doxorubicin

Additional file 1: Figure S1. MCF-7 & MDA-MB-231 cell lines transfected with FAM-labeled microRNAs. Fluorescence microscopy images of MCF-7 and MDA-MB-231 cells transfected with miR-154 mimic and miR inhibitor negative controls (NC) labeled with FAM. a) Evaluation of transfection efficiency under fluorescence and light microscope in MCF-7 cells transfected with FAM-labeled miR-154 mimic NC and miR-inhibitor NC. b) Evaluation of transfection efficiency, under fluorescence and light microscope, in MDA-MB-231 cells transfected with FAM-labeled miR-154 mimic NC and miR-inhibitor NC. The fluorescence microscopy images were obtained 24â h after transfection. Table S1. primer sequences for real-time PCR, and primer sequences used for amplification and cloning of NAMPT 3â ˛-UTR. The underlined sequences represent the restriction enzyme recognition sites (CTCGAG for XhoI and GCGGCCGC for NotI) added for cloning into psiCHECK-2.