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Effects of Bothrops asper snake venom on the expression of cyclooxygenases and production of prostaglandins by peritoneal leukocytes in vivo, and by isolated neutrophils and macrophages in vitro
- Source :
- Prostaglandins, Leukotrienes and Essential Fatty Acids; Volumen 80, Número 2-3. 2009, Kérwá, Universidad de Costa Rica, instacron:UCR
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- In this study, the ability of Bothrops asper snake venom (BaV) to increase the production of prostaglandins PGE2 and PGD2 was assessed in a mouse model in vivo and in inflammatory cells in vitro. In addition, the expressions of COX-1 and COX-2 were assessed. BaV induced an increment in the in vivo synthesis of PGE2 and PGD2, together with an enhanced expression of COX-2, but not of COX-1. However, enzymatic activities of COX-1 and COX-2 were increased. Incubation of isolated macrophages and neutrophils with a sub-cytotoxic concentration of BaV in vitro resulted in increased release of PGE2 and PGD2 by macrophages and PGE2 by neutrophils, concomitantly with an increment in the expression of COX-2, but not of COX-1 by both cell types. Our results demonstrate the ability of BaV to promote the expression of COX-2 and to induce the synthesis of proinflammatory prostaglandins. Macrophages and neutrophils may be important targets for this venom under in vivo situation. UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)
- Subjects :
- Male
Snake venom
Cell Survival
Neutrophils
Macrophage
Neutrophile
Clinical Biochemistry
Prostaglandin
Pharmacology
Biology
Dinoprostone
Proinflammatory cytokine
Mice
chemistry.chemical_compound
In vivo
Crotalid Venoms
Leukocytes
Animals
Ascitic Fluid
Bothrops
Peritoneal Cavity
Prostaglandin D2
Neutrophil
Membrane Proteins
Cell Biology
Cyclooxygenases
In vitro
chemistry
Eicosanoid
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Immunology
Biocatalysis
Cyclooxygenase 1
Macrophages, Peritoneal
Prostaglandins
lipids (amino acids, peptides, and proteins)
Injections, Intraperitoneal
Subjects
Details
- ISSN :
- 09523278
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Prostaglandins, Leukotrienes and Essential Fatty Acids
- Accession number :
- edsair.doi.dedup.....14c8af329a430019af137329c1456592