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Using in-situ environmental DNA sampling to detect the invasive New Zealand Mud Snail (Potamopyrgus antipodarum) in freshwaters
- Source :
- PeerJ, Vol 9, p e11835 (2021)
- Publication Year :
- 2021
- Publisher :
- PeerJ, 2021.
-
Abstract
- Environmental DNA (eDNA) detection of aquatic invasive species is currently at the forefront of aquatic conservation efforts because the methodology provides a cost effective and sensitive means to detect animals at low densities. Developments in eDNA technologies have improved detection probabilities for rare, indicator, and invasive species over the past decade. However, standard lab analysis can take days or weeks before results are available and is prohibitive when rapid management decisions are required for mitigation. Here, we investigated the performance of a real-time quantitative PCR system for on-site eDNA detection of New Zealand mud snails (Potamopyrgus antipodarum). Six sites in western Washington, USA were sampled using the rapid eDNA technique and traditional methods, with five samples per site. On-site eDNA detection of mud snails resulted in a 10% increase in positive sites (16/30 = 53% positive) relative to visual surveys (13/30 = 43% positive). In addition, positive associations were observed between mud snail eDNA concentration (eDNA copies per reaction) and the number of mud snail individuals at each site (R2 = 0.78). We show that the rapid on-site eDNA technology can be effective for detection and quantification of New Zealand mud snails in freshwaters. This on-site eDNA detection approach could possibly be used to initiate management protocols that allow for more rapid responses during the onset of biological invasions.
- Subjects :
- In situ
biology
General Neuroscience
Zoology
Sampling (statistics)
Environmental DNA
General Medicine
Snail
biology.organism_classification
General Biochemistry, Genetics and Molecular Biology
Invasive species
Molecular ecology
biology.animal
parasitic diseases
Medicine
Non-native snails
Biological invasions
General Agricultural and Biological Sciences
Potamopyrgus antipodarum
Subjects
Details
- ISSN :
- 21678359
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....14eb6db6edc9442157b4b0c8a5cd8450
- Full Text :
- https://doi.org/10.7717/peerj.11835