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Environmental metabarcoding reveals contrasting microbial communities at two poplar phytomanagement sites

Authors :
Olivier Girardclos
Benoît Valot
Damien Blaudez
Michel Chalot
Alexis Durand
Cyril Zappelini
Julie Foulon
Laboratoire Chrono-environnement ( LCE )
Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC )
Laboratoire Interdisciplinaire des Environnements Continentaux ( LIEC )
Université de Lorraine ( UL ) -Centre National de la Recherche Scientifique ( CNRS )
Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE)
Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)
Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo)
Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE)
Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Source :
Science of the Total Environment, Science of the Total Environment, Elsevier, 2016, 571, pp.1230-1240. 〈10.1016/j.scitotenv.2016.07.151〉, Science of the Total Environment, Elsevier, 2016, 571, pp.1230-1240. ⟨10.1016/j.scitotenv.2016.07.151⟩
Publication Year :
2016
Publisher :
HAL CCSD, 2016.

Abstract

The aim of the present study is to deepen the current understanding of the microbial communities at two poplar phytomanagement sites to reveal the environmental factors that drive the abundance, diversity and composition of microbial communities. A soil analysis revealed that the two soils displayed contrasting physico-chemical characteristics, with significant lower pH and higher Cd, Zn and Mn CaCl2-extractable fractions at Leforest site, compared with Pierrelaye site. The fungal and bacterial community profiles in the poplar roots and soils were assessed through Illumina MiSeq sequencing. Diversity indices and β-diversity measures illustrated that the root microbial communities were well separated from the soil microbial communities at both sites. A detailed study of the fungal composition showed that Ascomycota dominated the overall fungal communities on poplar soil, the root samples at Pierrelaye, and the unplanted soil at the experimental sites. Conversely, Basidiomycota accounted for a much higher percentage of the fungal community in poplar root samples from the Leforest site. The root bacterial communities were dominated by Alphaproteobacteria and Actinobacteria, and the soil samples were dominated by Alphaproteobacteria and Acidobacteria. The occurrence and dominance of the ectomycorrhizal community at Leforest but not at Pierrelaye is the major feature of our data set. Overall, ectomycorrhizal root symbionts appeared to be highly constrained by soil characteristics at the phytomanagement sites. Our data support the view that mycorrhizal inoculation is needed in highly stressed and nutrient-poor environments.

Details

Language :
English
ISSN :
00489697 and 18791026
Database :
OpenAIRE
Journal :
Science of the Total Environment, Science of the Total Environment, Elsevier, 2016, 571, pp.1230-1240. 〈10.1016/j.scitotenv.2016.07.151〉, Science of the Total Environment, Elsevier, 2016, 571, pp.1230-1240. ⟨10.1016/j.scitotenv.2016.07.151⟩
Accession number :
edsair.doi.dedup.....14f435d15533825a26f88202807a8bb3
Full Text :
https://doi.org/10.1016/j.scitotenv.2016.07.151〉