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Expression of selected cytochrome P450 isoforms and of cooperating enzymes in colorectal tissues in selected pathological conditions

Authors :
Danuta Plewka
Michał Morek
Edyta Bogunia
Tomasz Szczepanik
Czarosław Kijonka
Andrzej Plewka
Piotr Wittek
Source :
Pathology, research and practice. 210(4)
Publication Year :
2013

Abstract

The current interest in CYP expression in the colon results from its uniqueness as a target organ for cancer. To date, the CYP expression profiles in the colon have not yet been subject of comprehensive research. In this study, we investigated 40 patients with Crohn's disease, 40 with ulcerative colitis, and 40 healthy subjects as a control group. Colon tissues were fixed, dehydrated, cleared in xylene and embedded in paraffin. Sections were prepared from paraffin blocks for immunohistochemical staining with specific antibodies. We used antibodies to the human CYP1A1, CYP2B6, CYP2C9, CYP2E1 and CYP3A4 isoforms, as well as antibodies to the human glycoprotein P, glutathione-S transferase and antibody to the UDP-glucuronosyltransferase. The sections were stained immunohistochemically and examined using light microscopy. Cellular localization was determined, and computer image analysis was used. In all cases with Crohn's disease, the proteins studied showed at least a twofold expression. Ulcerative colitis showed a much weaker influence regarding the expression of the proteins studied but in case of CYP2C9 and UDP-glucuronosyltransferase, a decrease of expression was observed.

Details

ISSN :
16180631
Volume :
210
Issue :
4
Database :
OpenAIRE
Journal :
Pathology, research and practice
Accession number :
edsair.doi.dedup.....153434ee9eb9d62dc96d95b81c2b975c