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Expression of selected cytochrome P450 isoforms and of cooperating enzymes in colorectal tissues in selected pathological conditions
- Source :
- Pathology, research and practice. 210(4)
- Publication Year :
- 2013
-
Abstract
- The current interest in CYP expression in the colon results from its uniqueness as a target organ for cancer. To date, the CYP expression profiles in the colon have not yet been subject of comprehensive research. In this study, we investigated 40 patients with Crohn's disease, 40 with ulcerative colitis, and 40 healthy subjects as a control group. Colon tissues were fixed, dehydrated, cleared in xylene and embedded in paraffin. Sections were prepared from paraffin blocks for immunohistochemical staining with specific antibodies. We used antibodies to the human CYP1A1, CYP2B6, CYP2C9, CYP2E1 and CYP3A4 isoforms, as well as antibodies to the human glycoprotein P, glutathione-S transferase and antibody to the UDP-glucuronosyltransferase. The sections were stained immunohistochemically and examined using light microscopy. Cellular localization was determined, and computer image analysis was used. In all cases with Crohn's disease, the proteins studied showed at least a twofold expression. Ulcerative colitis showed a much weaker influence regarding the expression of the proteins studied but in case of CYP2C9 and UDP-glucuronosyltransferase, a decrease of expression was observed.
- Subjects :
- Gene isoform
Adult
Male
Pathology
medicine.medical_specialty
Pathology and Forensic Medicine
Crohn Disease
Cytochrome P-450 Enzyme System
medicine
Humans
Cellular localization
chemistry.chemical_classification
biology
Cancer
Cytochrome P450
Cell Biology
Middle Aged
medicine.disease
Ulcerative colitis
Immunohistochemistry
Isoenzymes
chemistry
biology.protein
Colitis, Ulcerative
Female
Antibody
Glycoprotein
Transcriptome
Subjects
Details
- ISSN :
- 16180631
- Volume :
- 210
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Pathology, research and practice
- Accession number :
- edsair.doi.dedup.....153434ee9eb9d62dc96d95b81c2b975c