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Analysis of metabolomic patterns in thoroughbreds before and after exercise
- Source :
- Asian-Australasian Journal of Animal Sciences, Vol 30, Iss 11, Pp 1633-1642 (2017), Asian-Australasian Journal of Animal Sciences, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(30): 11
- Publication Year :
- 2017
- Publisher :
- Asian-Australasian Association of Animal Production Societies, 2017.
-
Abstract
- Objective Evaluation of exercise effects in racehorses is important in horseracing industry and animal health care. In this study, we compared metabolic patterns between before and after exercise to screen metabolic biomarkers for exercise effects in thoroughbreds. Methods The concentration of metabolites in muscle, plasma, and urine was measured by 1H nuclear magnetic resonance (NMR) spectroscopy analysis and the relative metabolite levels in the three samples were compared between before and after exercise. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA) and variable important plots and t-test was used for basic statistical analysis. Results From 1H NMR spectroscopy analysis, 35, 25, and 34 metabolites were detected in the muscle, plasma, and urine. Aspartate, betaine, choline, cysteine, ethanol, and threonine were increased over 2-fold in the muscle; propionate and trimethylamine were increased over 2-fold in the plasma; and alanine, glycerol, inosine, lactate, and pyruvate were increased over 2-fold whereas acetoacetate, arginine, citrulline, creatine, glutamine, glutarate, hippurate, lysine, methionine, phenylacetylglycine, taurine, trigonelline, trimethylamine, and trimethylamine N-oxide were decreased below 0.5-fold in the urine. The OPLS-DA showed clear separation of the metabolic patterns before and after exercise in the muscle, plasma, and urine. Statistical analysis showed that after exercise, acetoacetate, arginine, glutamine, hippurate, phenylacetylglycine trimethylamine, trimethylamine N-oxide, and trigonelline were significantly decreased and alanine, glycerol, inosine, lactate, and pyruvate were significantly increased in the urine (p
- Subjects :
- 0301 basic medicine
Metabolic Analysis
medicine.medical_specialty
040301 veterinary sciences
Metabolite
lcsh:Animal biochemistry
Trimethylamine
Urine
Creatine
Article
0403 veterinary science
03 medical and health sciences
chemistry.chemical_compound
Trigonelline
Internal medicine
medicine
Choline
Exercise
lcsh:QP501-801
lcsh:SF1-1100
Racehorse
Thoroughbred
04 agricultural and veterinary sciences
Animal Biotechnology
Glutamine
030104 developmental biology
Endocrinology
chemistry
Animal Science and Zoology
Pyruvic acid
lcsh:Animal culture
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 19765517 and 10112367
- Volume :
- 30
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Asian-Australasian Journal of Animal Sciences
- Accession number :
- edsair.doi.dedup.....158a156503f5f4bae6c1fba9c357004b