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New Subgenotyping and Consensus Real-Time Reverse Transcription-PCR Assays for Hepatitis A Outbreak Surveillance
New Subgenotyping and Consensus Real-Time Reverse Transcription-PCR Assays for Hepatitis A Outbreak Surveillance
- Source :
- Journal of Clinical Microbiology. 57
- Publication Year :
- 2019
- Publisher :
- American Society for Microbiology, 2019.
-
Abstract
- Laboratory surveillance plays an important role in the detection and control of hepatitis A outbreaks and requires the application of rapid and accurate molecular diagnostic tools for hepatitis A virus (HAV) RNA detection, subgenotype identification, and sequence-based genotyping. We describe the development and validation of a triplex real-time, reverse transcription-PCR (triplex rRT-PCR) assay for the identification and discrimination of HAV subgenotypes IA, IB, and IIIA and a singleplex rRT-PCR assay designed to detect all HAV genotypes infecting humans. Overall, the accuracy, sensitivity, and specificity of the new assays were >97% for serum and plasma specimens collected during unrelated outbreaks of HAV in California and Michigan compared to a nested RT-PCR genotyping assay and the ISO 15216-1 rRT-PCR method for HAV detection. The new assays will permit the rapid detection of HAV RNA and discrimination among subgenotypes IA, IB, and IIIA in serum and plasma specimens, which will strengthen public health surveillance efforts for HAV outbreak detection and response.
- Subjects :
- Microbiology (medical)
Michigan
Genotype
Genotyping Techniques
viruses
Biology
Real-Time Polymerase Chain Reaction
Diagnostic tools
Sensitivity and Specificity
Rapid detection
California
Disease Outbreaks
Virology
medicine
Humans
Genotyping
Molecular Epidemiology
Reverse Transcriptase Polymerase Chain Reaction
virus diseases
Hepatitis A
Outbreak
medicine.disease
digestive system diseases
Hepatitis a virus
Reverse transcription polymerase chain reaction
Epidemiological Monitoring
Hepatitis A virus
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....15b44670e4a02967a708dede4b78bed1
- Full Text :
- https://doi.org/10.1128/jcm.00500-19