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Two-dimension antigenic dendrogram and phylogenetic tree of avian influenza virus H5N1
- Publication Year :
- 2012
- Publisher :
- Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1, 2012.
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Abstract
- The hemagglutination-inhibition (HI) titers of a panel of 25 mouse monoclonal antibodies (MAbs) against 44 isolates of highly pathogenic avian influenza virus H5N1 were determined. A two-dimensional antigenic dendrogram was constructed by hierarchical clustering of HI titers. Viruses with similar reactivity patterns were clustered horizontally, whereas MAbs were clustered vertically. In this 2-D dendrogram, with 40% similarity as a cut-off, four virus clusters and four MAbs clusters were identified. A phylogenetic tree based on the deduced amino acid sequence of the hemagglutinin gene of tested viruses was constructed and its topology was compared to the antigenic dendrogram. Interestingly, viruses with high genetic homology in the phylogenetic tree also had high similarity in their reactivity patterns, as indicated by their relatedness in the tree and close clustering in the dendrogram, respectively. However, the reverse and the converse were also true. Of the five pairs of viruses in the dendrogram with bootstrap values higher than 75, four pairs were in concordance with their genetic relatedness. However, one pair contained viruses belong to two distinct genetic clades. These results were discussed in the context of antigenic variation, genetic polymorphism, and the potential application of MAbs in antigenic analysis.<br />link_to_OA_fulltext
- Subjects :
- Microbiology (medical)
Monoclonal antibody
Turkeys
Immunology
Hemagglutinins, Viral
Hemagglutinins, Viral - chemistry - genetics - immunology
Biology
Antibodies, Viral
medicine.disease_cause
Microbiology
Article
Virus
Hierarchical clustering
Evolution, Molecular
Mice
Phylogenetics
Influenza, Human
Antigenic variation
medicine
Influenza A virus
Animals
Cluster Analysis
Humans
Immunology and Allergy
Amino Acid Sequence
Antigens, Viral
Phylogeny
Influenza in Birds - immunology - virology
Genetics
Influenza A Virus, H5N1 Subtype
Phylogenetic tree
Avian influenza virus H5N1
Dendrogram
Antibodies, Monoclonal
General Medicine
Hemagglutination Inhibition Tests
Influenza A Virus, H5N1 Subtype - genetics - immunology
Virology
Influenza A virus subtype H5N1
Infectious Diseases
Antigenic cluster
Antigens, Viral - chemistry - immunology
Influenza in Birds
Antigenic dendrogram
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....15c7ac72165442aac065d19b13c2f10f