Back to Search
Start Over
The Possible Role of Sclerostin in the Pathogenesis of Tympanosclerosis
- Source :
- Audiology and Neurotology. 26:102-110
- Publication Year :
- 2021
- Publisher :
- S. Karger AG, 2021.
-
Abstract
- Objective: The aim of this study was to investigate sclerostin (SOST) expression in a rat model of experimental tympanosclerosis (TS) and its possible role in the formation of TS. Materials and Methods: Thirty-four SD rats were randomly divided into 2 groups: experimental group (n = 17) and normal group (n = 17). The left tympanic cavities in the experimental group were inoculated with methicillin-resistant Staphylococcus aureus. The changes of tympanic membranes were examined and recorded under otoendoscope. Haematoxylin-eosin staining was adopted to detect the morphological changes in the tympanic membrane and middle ear mucosa. Immunohistochemistry and Western blot analysis were used to observe the expression of SOST, Wnt3a, β-catenin, and P-ERK1/2. Results: In the experimental group, sclerotic lesions were observed in 54.5% ears in the end of 6 weeks. Morphological changes such as mucosa incrassation, inflammatory cells infiltration, fibrous tissue proliferation, and interstitial tissue incrassation prominently appeared in the tympanic membrane and middle ear mucosa. SOST protein was mainly distributed in the cytoplasm of epithelial cells and gland cells, the expression of which increased significantly in the calcified experimental ears. In addition, expression levels of Wnt3a, β-catenin, and P-ERK1/2 increased significantly in the calcified group too. Conclusion: The upregulated expression level of SOST may be involved in the formation of TS, first, through the pro-phosphorylation of ERK1/2 in the inflammatory stage, and then through the enhancement of Wnt3a in the osteogenic stage.
- Subjects :
- Genetic Markers
Male
Methicillin-Resistant Staphylococcus aureus
Pathology
medicine.medical_specialty
Tympanic Membrane
Physiology
Ear, Middle
Rats, Sprague-Dawley
Pathogenesis
Speech and Hearing
chemistry.chemical_compound
Downregulation and upregulation
Western blot
medicine
Animals
Tympanosclerosis
beta Catenin
medicine.diagnostic_test
business.industry
Myringosclerosis
medicine.disease
Sensory Systems
Rats
Staining
Disease Models, Animal
Otorhinolaryngology
chemistry
Bone Morphogenetic Proteins
Immunohistochemistry
Sclerostin
business
Infiltration (medical)
Subjects
Details
- ISSN :
- 14219700 and 14203030
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Audiology and Neurotology
- Accession number :
- edsair.doi.dedup.....15c95657197716a1516610e77eb9542c