Figures S1-S5, Table S1 from Melanoma Extracellular Vesicles Generate Immunosuppressive Myeloid Cells by Upregulating PD-L1 via TLR4 Signaling

Figure S1 shows PD-L1 upregulation induced by EV that is mediated by NF-kB activation; Figure S2 shows an induction of PD-L1 and immunosuppressive capacity of myeloid cells through TLR signaling; Figure S3 presents characterization of EV derived from human melanoma cells; Figure S4 shows induction of PD-L1 expression on normal monocytes by melanoma EV; Figure S5 shows HSP86 expression in HT-144 human melanoma cells and HT-144-EV; Table S1 presents the sequences of primers for the quantitative real-time PCR